Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord
Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord
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DOI：
10.1016/j.jneuroim.2010.07.017
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发表时间：
2010-10-08
影响因子：
3.3
通讯作者：
Stein, Veronika M.
中科院分区：
文献类型：
--
作者：
Ensinger, Eva-Maria;Boekhoff, Theda M. A.;Stein, Veronika M.
小胶质细胞（中枢神经系统（CNS）的常驻免疫效应元件）表面分子表达和功能活性的调节差异可能为了解中枢神经系统内某些疾病的好发部位提供重要的见解。因此，使用密度梯度离心和流式细胞术在同质犬群中离体评估与健康中枢神经系统内不同地形区域相关的犬小胶质细胞，从大脑、颈椎和胸腰段脊髓中提取。免疫表型特征显示 B7-1、CD14、CD44、CD1c、CD18、CD11b 和 CD11c 存在生理区域差异。吞噬作用和活性氧生成都揭示了大脑、颈椎和胸腰段脊髓之间的差异。我们的结果强调，小胶质细胞在中枢神经系统内表现出生理地形区域差异。狗似乎是进一步研究小胶质细胞在脊髓局部病理状况中的作用的理想模型。 (c) 2010 Elsevier B.V. 保留所有权利。
Differences in the regulation of surface molecule expression and functional activity of microglia, the resident immune effector elements of the central nervous system (CNS), might give important insights into understanding the predilection sites of some diseases within the CNS. Therefore, canine microglial cells in relation to different topographical regions within the healthy CNS were evaluated ex vivo from the brain, cervical, and thoracolumbar spinal cord using density gradient centrifugation and flow cytometry in a homogenous dog population.Immunophenotypical characterization showed physiological regional differences for B7-1, CD14, CD44, CD1c, CD18, CD11b, and CD11c. Both, phagocytosis and ROS generation revealed differences between the brain, cervical, and thoracolumbar spinal cord.Our results emphasize that microglia displays physiological topographical regional differences within the CNS. The dog seems to be an ideal model to further investigate the role of microglia in focal pathological conditions of the spinal cord. (c) 2010 Elsevier B.V. All rights reserved.