The chemical genomic portrait of yeast: Uncovering a phenotype for all genes
The chemical genomic portrait of yeast: Uncovering a phenotype for all genes
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DOI：
10.1126/science.1150021
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发表时间：
2008-04-18
期刊：
影响因子：
56.9
通讯作者：
Giaever, Guri
中科院分区：
文献类型：
--
作者：
Hillenmeyer, Maureen E.;Fung, Eula;Giaever, Guri
遗传学的目的是了解基因型和表现型之间的关系。然而，由于大多数酵母基因的完全缺失（类似于80%）在丰富培养基中没有明显的表型后果，因此很难研究它们的功能。为了揭示基因组的这种非必需部分的表型，我们对酵母全基因组杂合和纯合缺失集合进行了1144次化学基因组测定，并量化了每种缺失菌株在化学或环境胁迫条件下的生长适应性。我们发现97%的基因缺失表现出可测量的生长表型，这表明几乎所有的基因在至少一种条件下对最佳生长都是必不可少的。
Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes (similar to 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole- genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.