Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium
Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium
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DOI：
10.1128/jb.168.3.1172-1179.1986
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发表时间：
1986-12
影响因子：
3.2
通讯作者：
S. Yamaguchi;S. Aizawa;T. Kihara;M. Isomura;Christopher J Jones;R. Macnab
中科院分区：
文献类型：
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作者：
S. Yamaguchi;S. Aizawa;T. Kihara;M. Isomura;Christopher J Jones;R. Macnab
flaAII。2、鼠伤寒沙门菌的flq和flaN基因对鞭毛马达的组装、旋转和逆时针开关很重要。麻痹和非趋化突变体受到选择压力以获得部分运动性和趋化性，由此产生的伪回复性的抑制突变被定位和分离。许多基因间抑制突变发生在另外两个基因中的一个。其他基因位于趋化系统的细胞质成分基因中，特别是cheY和cheZ；其中一个突变是在cheA基因和一个运动基因motB中发现的。三个fla基因之间的抑制是等位基因特异性的，许多假回复性基因要么是冷敏感的，要么是热敏感的。我们得出结论，FlaAII。2、flq和FlaN蛋白形成一个复合体，决定电机的逆时针或顺时针旋转方向，并参与将质子能量转化为旋转的机械功。这个开关复合体可能安装在鞭毛基体的基部，通过CheY和CheZ蛋白的结合，接收感觉信息并利用它来控制鞭毛的操作。
The flaAII.2, flaQ, and flaN genes of Salmonella typhimurium are important for assembly, rotation, and counterclockwise-clockwise switching of the flagellar motor. Paralyzed and nonchemotactic mutants were subjected to selection pressure for partial acquisition of motility and chemotaxis, and the suppressor mutations of the resulting pseudorevertants were mapped and isolated. Many of the intergenic suppressor mutations were in one of the other two genes. Others were in genes for cytoplasmic components of the chemotaxis system, notably cheY and cheZ; one of the mutations was found in the cheA gene and one in a motility gene, motB. Suppression among the three fla genes was allele specific, and many of the pseudorevertants were either cold sensitive or heat sensitive. We conclude that the FlaAII.2, FlaQ, and FlaN proteins form a complex which determines the rotational sense, either counterclockwise or clockwise, of the motor and also participates in the conversion of proton energy into mechanical work of rotation. This switch complex is probably mounted to the base of the flagellar basal body and, via binding of the CheY and CheZ proteins, receives sensory information and uses it to control flagellar operation.