Lactation Reduces Stress-Caused Dopaminergic Activity and Enhances GABAergic Activity in the Rat Medial Prefrontal Cortex
Lactation Reduces Stress-Caused Dopaminergic Activity and Enhances GABAergic Activity in the Rat Medial Prefrontal Cortex
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DOI：
10.1007/s12031-013-0104-7
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发表时间：
2014-04-01
影响因子：
3.1
通讯作者：
Guevara-Guzman, Rosalinda
中科院分区：
文献类型：
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作者：
Arriaga-Avila, Virginia;Martinez-Abundis, Eduardo;Guevara-Guzman, Rosalinda
观察束缚对哺乳期雌性Wistar大鼠内侧前额叶皮质(MPFC)多巴胺、GABA和谷氨酸释放的影响，以及对神经肽YY2、GABA受体和促肾上腺皮质激素释放因子(CRF)表达的影响。微透析实验结果显示，哺乳期大鼠的基础多巴胺和GABA浓度较高，但谷氨酸浓度不高。在处女动物中，制动导致多巴胺显著增加，而GABA没有变化，谷氨酸减少。在哺乳期动物中，抑制显著降低了多巴胺浓度，而与处女动物相比，GABA和谷氨酸浓度增加。结果发现，应激大鼠左侧mPFC中CRF、D_1和神经肽Y_Y2受体均有较高的表达，仅应激乳期大鼠左侧扣带回GABA免疫阳性细胞显著增加，而应激后处女大鼠左侧前庭皮质GABA免疫阳性细胞数显著减少。应激期间mPFC多巴胺细胞的抑制增加和神经肽Y Y2受体表达的下调可能解释了哺乳期动物CRF降低和对应激反应低下的原因。有趣的是，mPFC在压力调节中的参与似乎是片面的。
We investigated the effect of restraint on the release of dopamine, GABA and glutamate in the medial prefrontal cortex (mPFC) of lactating compared with virgin Wistar female rats; besides the expression of D1, neuropeptide Y Y2, GABA receptors and corticotropin-releasing factor (CRF). Results from microdialysis experiments showed that basal dopamine and GABA, but not glutamate, concentrations were higher in lactating rats. In virgin animals, immobilization caused significant increase in dopamine, whereas GABA was unchanged and glutamate reduced. In lactating animals, restrain significantly decreased dopamine concentrations and, in contrast to virgin animals, GABA and glutamate concentrations increased. We found a higher expression of CRF, as well as the D1 and neuropeptide Y Y2 receptors in the left mPFC of virgin stressed rats; also, only stressed lactating animals showed a significant increase in immunopositive cells to GABA in the left cingulate cortex; meanwhile, a significant decrease was measured in virgin rats after stress in the left prelimbic region. The increased inhibition of the mPFC dopamine cells during stress and the down-regulated expression of the neuropeptide Y Y2 receptor may explain the lower CRF and hyporesponse to stress measured in lactating animals. Interestingly, participation of mPFC in stress regulation seems to be lateralized.