Influence of thyroid status and growth hormone deficiency on ghrelin
Influence of thyroid status and growth hormone deficiency on ghrelin
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DOI：
10.1530/eje.0.1470159
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发表时间：
2002-07-01
影响因子：
5.8
通讯作者：
Dieguez, C
中科院分区：
文献类型：
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作者：
Caminos, JE;Seoane, LM;Dieguez, C
目的：探讨Ghrelin是否参与了甲状腺功能紊乱和GH缺乏时能量平衡的改变。设计：成年雄性SD大鼠分别给予赋形剂(正常甲状腺)、氨基三氮唑(甲状腺功能低下)和1-甲状腺素(甲状腺功能亢进)治疗，以评价甲状腺状态对Ghrelin的影响。观察GH对野生型(对照组)和GH缺乏型(矮小)Lewis大鼠Ghrelin的影响。Northern印迹法检测胃组织Ghrelin基因的表达。结果：甲状腺功能减退可导致胃Ghrelin基因表达水平升高(正常甲状腺：127.3+/-3.2%vs甲状腺功能低下：127.3+/-6.5%；P<；0.01)，而甲亢大鼠胃Ghrelin m RNA水平明显降低(70+/-5.4%；P<；0.01)。与此结果一致，甲状腺功能减退组循环血浆Ghrelin水平升高(正常甲状腺组：124+/-11pg/mlvs甲状腺功能减退组：262+/-39pg/ml；P<；0.01)，而甲亢组则降低(75+/-6pg/ml；P<；0.01)。在生长激素缺乏的实验模型上，我们发现胃Ghrelin mRNA水平(对照组：100+/-6%vs侏儒66+/-5.5%；P<；0.01)和循环血浆ghrelin水平(对照组：124+/-12pg/mlvs侏儒：124+/-12pg/mlvs侏儒)。81+/-7pg/ml；P<；0.01)。结论：本研究首次提供了Ghrelin基因表达受甲状腺激素和GH状态影响的证据，并进一步证明在这些病理生理状态下，Ghrelin可能在能量平衡和体重变化中发挥重要作用。
Objective: To assess whether some of the alterations in energy homeostasis present in thyroid function disorders and GH deficiency could be mediated by ghrelin.Design: To assess the influence of thyroid status on ghrelin, adult male Sprague-Dawley rats were treated with vehicle (euthyroid), amino-triazole (hypothyroid) or 1-thyroxine (hyperthyroid). The influence of GH on ghrelin was assessed in wild-type (control) and GH-deficient (dwarf) Lewis rats. Evaluation of gastric ghrelin mRNA expression in the stomach was carried out by Northern blot. Circulating levels of ghrelin were measured by radioimmunoassay.Results: Hypothyroidism resulted in an increase in gastric ghrelin mRNA levels (euthyroid: 100+/-3.2% vs hypothyroid: 127.3+/-6.5%; P < 0.01), being decreased in hyperthyroid rats (70+/-5.4%; P < 0.01). In keeping with these results, circulating plasma ghrelin levels were increased in hypothyroid (euthyroid: 124+/-11 pg/ml vs hypothyroid: 262+/-39 pg/ml; P < 0.01) and decreased in hyperthyroid rats (75+/-6 pg/ml; P < 0.01). Using an experimental model of GH deficiency, namely the dwarf rat, we found a decrease in gastric ghrelin mRNA levels (controls: 100+/-6% vs dwarf 66+/-5.5%; P < 0.01) and circulating plasma ghrelin levels (controls: 124+/-12 pg/ml vs dwarf. 81+/-7 pg/ml; P < 0.01).Conclusion: This study provides the first evidence that ghrelin gene expression is influenced by thyroid hormones and GH status and provides further evidence that ghrelin may play an important role in the alteration of energy homeostasis and body weight present in these pathophysiological states.