Metabolic characteristics of primary inspiratory and expiratory muscles in the dog.
Metabolic characteristics of primary inspiratory and expiratory muscles in the dog.
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狗初级吸气肌和呼气肌的代谢特征。
DOI：
10.1152/jappl.1994.77.5.2188
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Dodd,S
中科院分区：
文献类型：
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作者：
Powers,SK;Farkas,GA;Criswell,D;Herb,RA;Zambito,K;Dodd,S
这些实验检查了犬呼吸肌的代谢特性。因为肋隔膜（COD）、脚隔膜（CRD）、胸骨旁肋间肌（PI）、胸骨三角肌（TS）和腹横肌（TA）在狗的安静呼吸期间是活跃的，所以我们假设这些肌肉将具有不同的代谢谱（即，更高的氧化和抗氧化酶活性）与仅在增加的排泄要求下募集的排泄肌相比[例如，斜角肌（SC）和外斜肌（EO）]和运动肌[例如，三角肌（DEL）]。为了验证这一假设，肌肉样品从六个健康的成年狗和分析，以确定柠檬酸合酶（CS），磷酸果糖激酶（PFK），3-羟酰辅酶A脱氢酶（HADH），和超氧化物歧化酶（SOD）的活动。这些酶的活性被解释为代谢能力的相对量度，并且酶活性比被认为代表不同代谢途径之间的关系。结果表明，PI、COD、CRD和TS肌中CS和HADH活性显著高于其他肌中CS和HADH活性（P < 0.05）。具有最低CS、HADH和SOD活性的肌肉（即，SC、TA、EO、DEL）中PFK活性最高，PI、COD、CRD、TS中PFK/CS比值显著低于其它肌肉。这些数据支持犬PI、COD、CRD和TS在代谢上不同于其他关键排泄肌的观点。
These experiments examined the metabolic properties of the canine respiratory muscles. Because the costal diaphragm (COD), crural diaphragm (CRD), parasternal intercostals (PI), triangularis sterni (TS), and transversus abdominis (TA) are active during quite breathing in the dog, we hypothesized that these muscles would have different metabolic profiles (i.e., higher oxidative and antioxidant enzyme activities) compared with ventilatory muscles recruited only at increased ventilatory requirements [e.g., scalene (SC) and external oblique (EO)] and locomotor muscles [e.g., deltoid (DEL)]. To test this hypothesis, muscle samples were removed from six healthy adult dogs and analyzed to determine the activities of citrate synthase (CS), phosphofructokinase (PFK), 3-hydroxyacyl-CoA dehydrogenase (HADH), and superoxide dismutase (SOD). The activities of these enzymes were interpreted as relative measures of metabolic capacities, and enzyme activity ratios were considered as representing relationships between different metabolic pathways. Analysis revealed that CS and HADH activities were significantly higher (P < 0.05) in the PI, COD, CRD, and TS compared with those in all other muscles. Muscles with the lowest CS, HADH, and SOD activities (i.e., SC, TA, EO, DEL) generally had the highest PFK activities, Furthermore, the PFK/CS ratio was significantly lower in the PI, COD, CRD, and TS compared with that in all other muscles studied. These data support the notion that the canine PI, COD, CRD, and TS are metabolically different from other key ventilatory muscles.