Elucidation of some fragmentations of small peptides using sequential mass spectrometry on a hybrid instrument.
Elucidation of some fragmentations of small peptides using sequential mass spectrometry on a hybrid instrument.
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DOI：
10.1002/rcm.1290030704
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发表时间：
1989-07-01
期刊：
影响因子：
--
通讯作者：
Gaskell, S J
中科院分区：
文献类型：
--
作者：
Thorne, G C;Gaskell, S J
使用BEqQ结构的混合串联仪器对小肽进行了顺序子离子扫描分析。前体离子由B选择，并允许或诱导(通过高能碰撞激活)在E之前的区域分解。E与加速电压的解耦允许选择第一代子离子，同时为四极组件保持适当的浮动电压。被E选择的子离子进一步在Q中进行低能碰撞激活解离(CAD)，并通过扫描Q获得碎片离子谱。用顺序子离子扫描技术证实，(AY)和(BY)类型的‘内部’碎片是通过初始的Y型解离形成的。首次报道了质子化多肽(血管紧张素III)通过失去C-末端氨基酸残基而断裂，产生(B‘n+OH)离子。这一过程类似于前面描述的金属阳离子多肽。
Sequential daughter-ion-scanning analyses of small peptides have been performed using a hybrid tandem instrument of BEqQ configuration. Precursor ions are selected by B and allowed or induced (by high-energy collisional activation) to decompose in the region preceding E. Decoupling of E from the accelerating voltage permits the selection of the first-generation daughter ion whilst retaining appropriate float voltages for the quadrupole assemblies. The daughter ion selected by E is further subjected to low-energy collisional-activation dissociation (CAD) in q and the fragment-ion spectrum is obtained by scanning Q. The sequential daughter-ion-scanning technique has been used to establish that 'internal' fragments of the types, (AY') and (BY'), are formed via initial Y-type cleavage. Fragmentation of a protonated peptide (angiotensin III) by loss of the C-terminal amino acid residue, yielding a (B'n + OH) ion, is reported for the first time. This process is analogous to that previously described for metal-cationized peptides.