HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.
HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.
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DOI：
10.15252/embj.201797677
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发表时间：
2018-03-15
期刊：
影响因子：
--
通讯作者：
Earnshaw WC
中科院分区：
文献类型：
--
作者：
Ruppert JG;Samejima K;Platani M;Molina O;Kimura H;Jeyaprakash AA;Ohta S;Earnshaw WC
在有丝分裂过程中，染色体过客复合物（CPC）通过与H3 T3 ph和Sgo 1结合而导向着丝粒。异染色质蛋白1α（HP 1 α）是否以及如何影响CPC在有丝分裂进入过程中的定位和功能尚不清楚。在这里，我们通过将其融合到CENP-B DNA结合结构域来改变HP 1 α动力学。拴系的HP 1强烈招募CPC，使运动舞蹈-微管相互作用不稳定并激活纺锤体组装检查点。在有丝分裂退出期间，系留的HP 1在着丝粒处捕获活性CPC。这些HP 1-CPC簇在随后的细胞周期中保持催化活性。我们还检测内源性HP 1和CPC之间的相互作用在G2。HP 1 α和HP 1 γ在CDK 1非依赖性过程中协同募集CPC至活性灶。用Fab片段进行活细胞追踪揭示，H3 S10 ph在H3 T3被Haspin激酶磷酸化之前很好地出现。我们的研究结果表明，在极光B介导的H3 S10磷酸化从染色质释放HP 1并允许依赖于H3 T3 ph和Sgo 1的途径将CPC重定向到有丝分裂的着丝粒之前，HP 1可能在G2的着丝粒异染色质处浓缩并激活CPC。
The chromosomal passenger complex (CPC) is directed to centromeres during mitosis via binding to H3T3ph and Sgo1. Whether and how heterochromatin protein 1α (HP1α) influences CPC localisation and function during mitotic entry is less clear. Here, we alter HP1α dynamics by fusing it to a CENP‐B DNA‐binding domain. Tethered HP1 strongly recruits the CPC, destabilising kinetochore–microtubule interactions and activating the spindle assembly checkpoint. During mitotic exit, the tethered HP1 traps active CPC at centromeres. These HP1‐CPC clusters remain catalytically active throughout the subsequent cell cycle. We also detect interactions between endogenous HP1 and the CPC during G2. HP1α and HP1γ cooperate to recruit the CPC to active foci in a CDK1‐independent process. Live cell tracking with Fab fragments reveals that H3S10ph appears well before H3T3 is phosphorylated by Haspin kinase. Our results suggest that HP1 may concentrate and activate the CPC at centromeric heterochromatin in G2 before Aurora B‐mediated phosphorylation of H3S10 releases HP1 from chromatin and allows pathways dependent on H3T3ph and Sgo1 to redirect the CPC to mitotic centromeres.
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哺乳动物 HP1 同工酶在异染色质结构和组织中发挥特殊作用
影响因子：
8.8
作者：
Bosch-Presegue, Laia;Raurell-Vila, Helena;Vaquero, Alejandro
通讯作者：
Vaquero, Alejandro
影响因子：
64.8
作者：
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通讯作者：
Kapoor, Tarun M.
影响因子：
4
作者：
Hayakawa, T;Haraguchi, T;Hiraoka, Y
通讯作者：
Hiraoka, Y
DOI：
10.1083/jcb.200404001
发表时间：
2004-07-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gassmann R;Carvalho A;Henzing AJ;Ruchaud S;Hudson DF;Honda R;Nigg EA;Gerloff DL;Earnshaw WC
通讯作者：
Earnshaw WC
DOI：
10.1111/j.1432-1033.1978.tb12135.x
发表时间：
1978-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GURLEY, LR;DANNA, JA;TOBEY, RA
通讯作者：
TOBEY, RA