Mutualistic dinoflagellates with big disparities in ribosomal DNA variation may confound estimates of symbiont diversity and ecology in the jellyfish Cotylorhiza tuberculata
Mutualistic dinoflagellates with big disparities in ribosomal DNA variation may confound estimates of symbiont diversity and ecology in the jellyfish Cotylorhiza tuberculata
复制标题
核糖体 DNA 变异差异巨大的互惠甲藻可能会混淆水母 Cotylorhiza tuberculata 中共生体多样性和生态学的估计
DOI：
10.1007/s13199-022-00880-x
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发表时间：
2022
期刊：
影响因子：
2.5
通讯作者：
Wiedenmann, Joerg
中科院分区：
文献类型：
--
作者：
LaJeunesse, Todd C.;Casado-Amezúa, Pilar;Hume, Benjamin C.;Butler, Caleb C.;Mordret, Solenn;Piredda, Roberta;De Luca, Pasquale;Pannone, Raimondo;Sarno, Diana;Wiedenmann, Joerg
对互惠甲藻的准确鉴定对于了解它们与动物互惠的生理、生态学和进化至关重要。Cotylorhiza tubrulata(Macri 1778)是地中海特有的一种常见的脊椎动物，部分依赖内共生甲藻生存和生长。为了进一步研究与这些动物相关的共生体的多样性，我们分析了FC的标本。结核是从地中海西部和公共水族馆采集的，使用ITS2rDNA的下一代测序和S rRNA和线粒体cob基因的部分Sanger直接测序的组合。在我们对NGS数据的分析中，我们表征了两个诊断ITS2图谱。结合来自其他遗传标记的信息，每个剖面对应于一个单一的共生体物种，而不是有时推断的不同的群落组合。嗜精神性布鲁氏菌在所有标本中都很常见，而水生孢子虫在许多个体中的丰度较低。B的核糖体阵列。嗜心菌是高度异质性的，包含在本研究中获得的所有样本中以相同的相对比例发现的 ~ 15共生序列变体，而基因组中的核糖体阵列为OFP。水母的同源性相对较高，主要由一个丰富的序列变异所代表。这种对rDNA数据的精确解释提高了对这些互惠生物的生态学和进化的理解。Cotylorhiza tuurizata与来自不同属的甲藻共生体的关联与之前的发现一致，表明具有不同生态位的进化分歧的共生体能够更好地共生。
The precise identification of mutualistic dinoflagellates is critical for understanding the physiology, ecology and evolution of their mutualisms with animals.Cotylorhiza tuberculata(Macri 1778) is a common scyphozoan endemic to the Mediterranean Sea and relies in part on endosymbiotic dinoflagellates (zooxanthellae) for survival and growth. To further study the diversity of symbionts associated with these animals, we analyzed specimens ofC. tuberculatacollected across the western Mediterranean Sea and from public aquaria, using a combination of next generation sequencing (NGS) of ITS2 rDNA and direct Sanger sequencing of partial 28 S rRNA and mitochondrial cob genes. Two diagnostic ITS2 profiles were characterized during our analysis of NGS data. Combined with information from additional genetic markers, each profile corresponds to a single species of symbiont, not diverse community assemblages as are sometimes inferred.Breviolum psygmophilumwas common in all specimens, whilePhilozoon medusarumoccurred at lower abundances in many individuals. The ribosomal array ofB. psygmophilumwas highly heterogeneous and contained ~ 15 co-occurring sequence variants found in the same relative proportions across all samples obtained in this study, while the ribosomal array in the genomes ofP. medusarumwas relatively homogeneous represented mostly by one abundant sequence variant. This precise interpretation of rDNA data improves understanding of the ecology and evolution of these mutualisms.Cotylorhiza tuberculata’s association with dinoflagellate symbionts from different genera is consistent with previous findings and suggests that evolutionary divergent symbionts with dissimilar niches are better able to coexistin hospite.