Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice
Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice
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DOI：
10.1093/ejo/cjq124
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发表时间：
2011-10-01
影响因子：
2.6
通讯作者：
Tanne, K.
中科院分区：
文献类型：
--
作者：
Marquez Hernandez, R. A.;Ohtani, J.;Tanne, K.
性激素对骨骼生长很重要。然而，性激素受体影响骨骼生长的机制尚不清楚。在正畸治疗中，需要开发一种骨成熟指标来准确预测生长的开始和结束。这一指标可能是通过筛选性激素而开发出来的。本研究以5日龄C57BL/6J小鼠为实验对象，探讨各性激素受体在新生小鼠骨生长中的作用。40只小鼠接受了切除(ORX)、卵巢切除(OVX)或假手术。术后1周取股骨和下颌骨进行免疫组织化学染色。或者，每天给80只小鼠注射雌激素受体α(ERα)、β受体(ERβ)或雄激素受体(AR)拮抗剂。第一次注射后一周，拍摄股骨和下颌骨的X线片并进行测量。用方差分析和成对比较(Fisher)检验各组测量值的差异。在假手术的雄性和雌性小鼠中，在所有实验期间ERβ比ERα和AR更显著。在整个实验过程中，与假手术对照组相比，ORX组和OVX组所有受体的表达均显著降低。此外，注射ERβ拮抗剂组的股骨和下颌骨生长受到显著影响，任何性激素缺乏都会导致骨生长减慢。特别是，ERβ的紊乱会在雄性和雌性小鼠出生后立即产生更大的异常。
Sex hormones are important for bone growth. However, the mechanism by which sex hormone receptors influence bone growth remains unclear. In orthodontic treatment, there is a need to develop an indicator of bone maturity to accurately predict the beginning and end of growth. This indicator might be developed from the screening of sex hormones. The purpose of this study was to investigate the role of each sex hormone receptor on bone growth in newborn mice.Five-day-old C57BL/6J mice were used in this experiment. Forty mice underwent an orchiectomy (ORX), ovariectomy (OVX), or sham surgery. One week after surgery, the femur and the mandible were resected for immunohistochemical staining. Alternatively, 80 mice were daily injected with antagonist against receptors oestrogen alpha (ER alpha), beta (ER beta), or androgen receptor (AR). One week after the first injection, radiographs of the femur and mandible were taken and then measured. Analysis of variance and pairwise comparisons (Fisher) were performed to examine the differences in values measured among the groupsIn the sham-operated male and female mice, ER beta was found to be more prominent than ER alpha and AR during all experimental periods. In the ORX and OVX groups, the expressions of all receptors were significantly reduced in comparison with the sham-operated control group throughout the experiment. Moreover, femur and mandibular growth were significantly affected in the group injected with ER beta antagonist.The deficiency of any sex hormone leads to reduced bone growth. In particular, a disturbance in ER beta produces a greater aberrance in both male and female mice immediately after birth.