Application of radioactive precursors for the evaluation of sensitivity of cancer cells to anticancer drugs.
Application of radioactive precursors for the evaluation of sensitivity of cancer cells to anticancer drugs.
复制标题
放射性前体在评估癌细胞对抗癌药物敏感性中的应用。
作者：
H. Ichihashi;T. Kondo;S. Sakakibara;S. Akiyama;T. Watanabe
建立了一种体外药敏试验来评估药物对癌细胞的致死作用。癌细胞是在放射性前体和抗癌药物存在的情况下培养的。药物效应是通过前体掺入DNA、RNA和蛋白质的速率的变化来估计的。微滴定板和多台自动细胞收割机使处理大量样品变得可行。放射性前体的掺入与药物诱导的细胞杀伤力密切相关。本试验的结果也与小鼠肿瘤的体内消退相关。这项测试似乎比其他类似的体外细胞杀伤力测试更可靠。为了用于临床研究，简化了测试板，并对25种人类癌症进行了测试。在80%的观察中，体外结果与临床结果呈正相关。
An in vitro drug sensitivity test was developed to estimate the lethal effects of drugs on cancer cells. Cancer cells were incubated in the presence of radioactive precursors and anticancer drugs. The drug effects were estimated from the changes in rates of incorporation of precursors into DNA, RNA, and protein. A microtiter plate and a multiple automatic cell harvester made feasible handling of a large number of samples. Incorporation of radioactive precursors was well correlated with drug-induced cell lethality. The results of this test were also correlated with in vivo regression of tumors of mice. This test appeared to be more reliable than other similar tests of cell lethality in vitro. For utilization in clinical studies, a test plate was simplified and 25 human cancers were tested. The in vitro results demonstrated a positive correlation with clinical results in 80% of the observations.