Rapid wavelength-swept spectrally encoded confocal microscopy
Rapid wavelength-swept spectrally encoded confocal microscopy
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DOI：
10.1364/opex.13.008214
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发表时间：
2005-10-03
期刊：
影响因子：
3.8
通讯作者：
Tearney, GJ
中科院分区：
文献类型：
--
作者：
Boudoux, C;Yun, SH;Tearney, GJ
光谱编码共焦显微镜（SECM）是一种允许通过窄直径光纤探针的限制进行共焦显微镜检查的技术。我们提出了一种新的方案，其中使用快速波长扫描源进行SECM。该系统允许以高达30帧/秒的速率采集大视场图像。离体获得的分辨率目标和组织标本的图像表现出高的横向（1.4 μ m）和轴向（6 μ m）分辨率。人体皮肤的成像是在体内进行的深度达350 μ m，允许细胞和亚细胞的细节被可视化在真实的时间。(c)2005年，美国光学学会。
Spectrally encoded confocal microscopy (SECM) is a technique that allows confocal microscopy to be performed through the confines of a narrow diameter optical fiber probe. We present a novel scheme for performing SECM in which a rapid wavelength swept source is used. The system allows large field of view images to be acquired at rates up to 30 frames/second. Images of resolution targets and tissue specimens acquired ex vivo demonstrate high lateral (1.4 mu m) and axial (6 mu m) resolution. Imaging of human skin was performed in vivo at depths of up to 350 mu m, allowing cellular and sub-cellular details to be visualized in real time. (c) 2005 Optical Society of America.