DIFFERENT KS-VALUES FOR HYDROGEN OF METHANOGENIC BACTERIA AND SULFATE REDUCING BACTERIA - AN EXPLANATION FOR THE APPARENT INHIBITION OF METHANOGENESIS BY SULFATE
DIFFERENT KS-VALUES FOR HYDROGEN OF METHANOGENIC BACTERIA AND SULFATE REDUCING BACTERIA - AN EXPLANATION FOR THE APPARENT INHIBITION OF METHANOGENESIS BY SULFATE
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DOI：
10.1007/bf00405893
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发表时间：
1982-01-01
影响因子：
2.8
通讯作者：
THAUER, RK
中科院分区：
文献类型：
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作者：
KRISTJANSSON, JK;SCHONHEIT, P;THAUER, RK
Desulfovibrio vulgaris (Marburg) 和 Methanobrevibacter arboriphilus (AZ) 是厌氧污水污泥细菌，分别以 H2 加硫酸盐和 H2 加 CO2 作为唯一能源生长。 H2 的表观 Ks 值确定为.apprx。硫酸盐还原菌为1μM，产甲烷菌为6μM。在 2 种细菌的混合细胞悬浮液中（调整至等于 Vmax），当 H2 供应受到速率限制时，D. vulgaris 的 H2 消耗速率是 M. arboriphilus 的 5 倍。产甲烷作用的明显抑制与根据 H2 的不同 Ks 值预期的顺序相同。底物亲和力的差异可以解释富含硫酸盐的环境中来自H 2 和CO 2 的产甲烷作用的抑制，其中H 2 浓度远低于5μM。
Desulfovibrio vulgaris (Marburg) and Methanobrevibacter arboriphilus (AZ) are anaerobic sewage sludge bacteria which grow on H2 plus sulfate and H2 plus CO2 as sole energy sources, respectively. The apparent Ks values for H2 were determined to be .apprx. 1 .mu.M for the sulfate reducing bacterium and 6 .mu.M for the methanogenic bacterium. In mixed cell suspensions of the 2 bacteria (adjusted to equal Vmax), the rate of H2 consumption by D. vulgaris was 5 times that of M. arboriphilus, when the H2 supply was rate limiting. the apparent inhibition of methanogenesis was of the same order as expected from the different Ks values for H2. Difference in substrate affinities can account for the inhibition of methanogenesis from H2 and CO2 in sulfate-rich environments, where the H2 concentration is well below 5 .mu.M.