Characterization of elemental distribution across human dentin-enamel junction by scanning electron microscopy with energy-dispersive X-ray spectroscopy.
Characterization of elemental distribution across human dentin-enamel junction by scanning electron microscopy with energy-dispersive X-ray spectroscopy.
复制标题
通过扫描电子显微镜和能量色散 X 射线光谱仪表征人体牙本质 - 牙釉质交界处的元素分布。
DOI:
10.1002/jemt.23648
复制
发表时间:
2021
影响因子:
2.5
通讯作者:
Wang,Yong
中科院分区:
文献类型:
--
作者:
Wang,Rong;Zhao,Donggao;Wang,Yong
The human dentin‐enamel junction (DEJ) is a natural junction that unites two dissimilar mineralized tissues in the human tooth: enamel and dentin. DEJ plays a critical role in maintaining structural and functional integrity of the tooth. However, its structure, chemical composition and function remain unclear and controversial. Systematic investigation of elemental distribution across human DEJ is still lacking in the literature. This study aimed to investigate the elemental distributions of Ca, P, O, C, N, Na, and Mg across the DEJ of human teeth using scanning electron microscope with energy dispersive spectroscopy of X‐ray. The results revealed abrupt changes in the distributions of six elements (C, N, Ca, P, Na, and Mg) across the DEJ. Specifically, the four mineral elements showed similar level of change in distribution, with Ca, P, Na decreasing while Mg increasing by 21%–25% from enamel to dentin side of the DEJ. The two organic elements C and N showed much larger changes in distribution, with C increasing by ~150% and N increasing by ~270% from enamel to dentin side of the DEJ. The slope of the distribution curves across the DEJ was estimated to be ~2 μm in width and coincided with the phase intermixing of the micro‐scallop structure of the DEJ.