Increased lipolysis but diminished gene expression of lipases in subcutaneous adipose tissue of healthy young males with intrauterine growth retardation
Increased lipolysis but diminished gene expression of lipases in subcutaneous adipose tissue of healthy young males with intrauterine growth retardation
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DOI：
10.1152/japplphysiol.00960.2011
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发表时间：
2011-12-01
影响因子：
3.3
通讯作者：
Stallknecht, Bente
中科院分区：
文献类型：
--
作者：
Hojbjerre, Lise;Alibegovic, Amra C.;Stallknecht, Bente
HojbJerre L，Alibegovic AC，Sonne MP，Dela F，Vaag A，Bruun JM，Stallkeck B。子宫内生长迟缓健康年轻男性皮下脂肪组织脂肪分解增加，脂肪酶基因表达减少。应用生理学杂志111：1863-1870,2011。2011年9月8日首次发表；当暴露在久坐的西方生活方式中时，doi:10.1152/japplphysiol.00960.2011.-Intrauterine生长迟缓与成年人的中心脂肪分布和患2型糖尿病的风险有关。脂肪分解增加是IUGR受试者早期代谢缺陷，但涉及的部位和分子机制尚不清楚。20名IUGR和20名对照组(CON)在卧床前和卧床后10天分别用葡萄糖钳夹技术和微透析法测定体内代谢，并用Xe-133清洗技术测定腹部和股骨皮下脂肪组织的血流量。此外，在SCAAT的活检组织中对脂肪酶的mRNA表达进行了评估。尽管脂肪酶基因表达明显降低，但IUGR患者SCAAT中的脂解作用显著高于CON患者。在SCAAT和SCFAT中，IUGR的血流量均高于CON。全身胰岛素敏感性组间无差异，卧床休息后胰岛素敏感性降低。卧床后，IUGR组的SCAAT脂解作用仍高于CON组，而SCFAT脂解作用在CON组减少，但IUGR组无明显变化。在发生全身胰岛素抵抗之前，患有IUGR的年轻男性的特征是体内脂肪组织脂肪分解和血流增加，与CON相比，脂肪酶的表达反常地减少，10天的身体不活动突出了基线结果。IUGR受试者表现出脂肪组织代谢的原发缺陷。
Hojbjerre L, Alibegovic AC, Sonne MP, Dela F, Vaag A, Bruun JM, Stallknecht B. Increased lipolysis but diminished gene expression of lipases in subcutaneous adipose tissue of healthy young males with intrauterine growth retardation. J Appl Physiol 111: 1863-1870, 2011. First published September 8, 2011; doi:10.1152/japplphysiol.00960.2011.-Intrauterine growth retardation (IUGR) is associated with a central fat distribution and risk of developing type 2 diabetes in adults when exposed to a sedentary Western lifestyle. Increased lipolysis is an early defect of metabolism in IUGR subjects, but the sites and molecular mechanisms involved are unknown. Twenty IUGR and 20 control (CON) subjects, aged 20-30 years, were studied before and after 10 days of bed rest using the glucose clamp technique combined with measurements of in vivo metabolism by microdialysis technique and blood flow by Xe-133 washout technique in subcutaneous abdominal (SCAAT) and femoral (SCFAT) adipose tissue. Additionally, mRNA expression of lipases was evaluated in biopsies from SCAAT. Lipolysis in SCAAT was substantially higher in IUGR than in CON subjects despite markedly lower mRNA expression of lipases. Blood flow was higher in IUGR compared with CON in both SCAAT and SCFAT. Whole body insulin sensitivity did not differ between groups and decreased after bed rest. After bed rest, SCAAT lipolysis remained higher in IUGR compared with CON, and SCFAT lipolysis decreased in CON but not in IUGR. Prior to the development of whole body insulin resistance, young men with IUGR are characterized by increased in vivo adipose tissue lipolysis and blood flow with a paradoxically decreased expression of lipases compared with CON, and 10 days of physical inactivity underlined the baseline findings. Subjects with IUGR exhibit primary defects in adipose tissue metabolism.