Diversity and distribution of anammox bacteria in water column and sediments of the Eastern Indian Ocean
Diversity and distribution of anammox bacteria in water column and sediments of the Eastern Indian Ocean
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东印度洋水体和沉积物中厌氧氨氧化细菌的多样性和分布
DOI：
10.1016/j.ibiod.2018.05.015
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发表时间：
2018
影响因子：
4.8
通讯作者：
Sun Jun
中科院分区：
文献类型：
--
作者：
Qian Gang;Wang Jing;Kan Jinjun;Zhang Xiaodong;Xia Zhiqiang;Zhang Xuecheng;Miao Yanyi;Sun Jun
厌氧氨氧化（anammox）在全球氮素循环中的重要性已得到广泛认可。然而，它们在东印度洋（EIO）的分布仍然未知。以16 SrRNA基因为靶基因，采用克隆文库和实时定量PCR（qPCR）技术，研究了从0-2000 m水体（7个深度）到表层沉积物中厌氧氨氧化细菌的多样性和丰度。结果表明，该海域的水体和沉积物中均广泛分布有厌氧氨氧化细菌，其中以Scalindua sp.组合为优势类群。水体中厌氧氨氧化细菌的多样性较高，但丰度（基于16 S rRNA基因的拷贝数）低于相应的沉积物样品。非度量多维尺度分析表明不同的空间格局（水平和垂直）：孟加拉湾和赤道的样本显示出强烈的分层在不同的层，并从其他两个地区不同。典范对应分析表明，溶解氧、温度和硝酸盐是影响厌氧氨氧化菌分布的主要环境因子。这是第一次详细的群落结构和分布的厌氧氨氧化细菌在EIO的调查，和结果是一致的（i）沉积的厌氧氨氧化细菌对脱氮的潜在贡献，（ii）通过海洋的厌氧氨氧化细菌多样性的扩散。
Anaerobic ammonium oxidization (anammox) has been widely recognized for its importance in global nitrogen cycle. While, their distributions in the Eastern Indian Ocean (EIO) still remain unknown. This study investigated the diversity and abundance of anammox bacteria from water column (0–2000 m with seven depths) down to the surface sediments in the EIO targeting 16S rRNA gene by using clone library and quantitative real-time PCR (qPCR) analysis. Results showed a wide distribution of anammox bacteria in both water column and sediments in the EIO, with theCandidatusScalindua sp. assemblage as the predominant group among the anammox communities. The diversity of anammox bacteria was higher in water column, but abundances (based on copy numbers of 16S rRNA gene) were lower than the corresponding sediment samples. Non-metric multidimensional scaling analysis demonstrated distinct spatial patterns (both horizontal and vertical): samples from the Bay of Bengal and the Equator showed strong stratification in different layers and were distinct from other two regions. Canonical correspondence analysis indicated that dissolved oxygen, temperature, and nitrate were the major environmental factors shaping the distribution of anammox bacteria. This is the first investigation on detailed community structure and distribution of anammox bacteria in the EIO, and the results are consistent with (i) potential contribution of sedimentary anammox bacteria towards nitrogen removal, and (ii) dispersal of anammox bacteria diversity via the ocean.