Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis-A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort.
Potential Muscle-Related Biomarkers in Predicting Curve Progression to the Surgical Threshold in Adolescent Idiopathic Scoliosis-A Pilot Proteomic Study Comparing Four Non-Progressive vs. Four Progressive Patients vs. A Control Cohort.
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预测青少年特发性脊柱侧凸曲线进展至手术阈值的潜在肌肉相关生物标志物——一项比较四名非进展患者、四名进展患者与对照队列的蛋白质组学试验研究
DOI：
10.3390/jcm10214927
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发表时间：
2021-10-25
影响因子：
3.9
通讯作者：
Lee WYW
中科院分区：
文献类型：
--
作者：
Wang Y;Chen H;Zhang J;Lam TP;Hung ALH;Cheng JCY;Lee WYW
以往的研究报道了青少年特发性脊柱侧凸（AIS）患者的肌肉形态和功能异常。为了回答这种异常是否可以反映在他们的循环和他们的临床意义，预测曲线进展到手术阈值，这项初步研究探讨了基线肌肉相关蛋白的存在及其与曲线进展的关联。在AIS首次临床访视时采集血浆样本，根据6年随访中的Cobb角将患者分为非进展组或进展组（N = 4和4），年龄和性别匹配的健康受试者（N = 50）。然后，对样品进行相对和绝对定量（iTRAQ）的同量异位标签，以全面比较非靶向蛋白质表达。71个差异表达蛋白（DEPs）被发现在进行性AIS中升高。功能分析表明，其中18个在肌肉中表达，并在肌肉活动中发挥重要作用。在肌肉相关DEP中，α-肌动蛋白在进行性/非进行性组中的倍数变化最高。这项初步研究首先表明，进行性AIS中肌肉结构蛋白的循环水平较高，表明微观水平上结构损伤的可能性及其与进展至手术阈值的相关性。进一步的研究，更大的样本量是必要的，以验证这些新的候选人的早期诊断和预测进展。
Previous studies have reported abnormal muscle morphology and functions in patients with adolescent idiopathic scoliosis (AIS). To answer whether such abnormalities could be reflected in their circulation and their clinical implication for predicting curve progression to the surgical threshold, this preliminary study explored the presence of baseline muscle-related proteins and their association with curve progression. Plasma samples were collected at the first clinical visit for AIS, with patients divided into non-progressive or progressive groups (N = four and four) according to their Cobb angle in six-year follow-ups, with age- and sex-matched healthy subjects (N = 50). Then, the samples were subjected to isobaric tags for relative and absolute quantitation (iTRAQ) for global comparison of untargeted protein expression. Seventy-one differentially expressed proteins (DEPs) were found elevated in progressive AIS. Functional analysis showed that 18 of these are expressed in muscles and play an essential role in muscle activities. Among the muscle-related DEPs, α-actin had the highest fold change in progressive/non-progressive groups. This preliminary study firstly suggested higher circulating levels of muscle structural proteins in progressive AIS, indicating the likelihood of structural damage at the microscopic level and its association with progression to the surgical threshold. Further studies with larger sample sizes are warranted to validate these novel candidates for early diagnosis and predicting progression.
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影响因子：
24.3
作者：
Martinez-Llorens, J.;Ramirez, M.;Gea, J.
通讯作者：
Gea, J.
影响因子：
2.9
作者：
Diz, Angel P.;Truebano, Manuela;Skibinski, David O. F.
通讯作者：
Skibinski, David O. F.
DOI：
10.1007/s005860050077
发表时间：
1998-01-01
期刊：
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
影响因子：
--
作者：
Mannion, A F;Meier, M;Muntener, M
通讯作者：
Muntener, M
DOI：
10.2106/00004623-199408000-00009
发表时间：
1994-08-01
影响因子：
5.3
作者：
KINDSFATER, K;LOWE, T;AKMAKJIAN, J
通讯作者：
AKMAKJIAN, J
影响因子：
2.3
作者：
Chan, YL;Cheng, JCY;Metreweli, C
通讯作者：
Metreweli, C