Long.-term keratocyte deficits in the corneal stroma after LASIK
Long.-term keratocyte deficits in the corneal stroma after LASIK
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DOI：
10.1016/j.ophtha.2003.10.027
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发表时间：
2004-07-01
期刊：
影响因子：
13.7
通讯作者：
Bourne, WM
中科院分区：
文献类型：
--
作者：
Erie, JC;Nau, CB;Bourne, WM
目的：研究准分子激光原位角膜磨镶术(LASIK)术后3年内角膜细胞密度的变化。设计：前瞻性、非随机、对照试验。参与者：11例(17只眼)接受LASIK手术，术后1、3、6、12、24、36个月用共聚焦显微镜检查屈光度在-2.0~-11.0D之间(平均-6.56+/-2.44)。戴面具的观察者手动对类似于角质细胞核的明亮物体进行计数。细胞密度测定在基质瓣的前半部分和后半部分，100微米厚的层的前半部分和后半部分(逆切削层)，以及基质的后三分之一。术前分析中省略了被消融的间质区域(LASIK术后1个月的测量)。主要观察指标：角膜细胞密度。结果：LASIK术前、术后1个月、术后1个月、术后1个月，角膜基质瓣前、后基质层和前层角膜基质细胞密度分别为34818+/-5108个/mm(3个)、25390+/-4045个/mm(3个)和21328+/-2980个/mm(3个)，下降了14%~20%(P
Purpose: To determine changes in keratocyte density up to 3 years after LASIK.Design: Prospective, nonrandomized, comparative trial.Participants: Seventeen eyes of 11 patients received LASIK with a planned 180-mum flap to correct refractive errors between -2.0 diopters (D) and -11.0 D (mean, -6.56+/-2.44).Methods: Corneas were examined by using confocal microscopy before LASIK and 1, 3, 6, 12, 24, and 36 months after LASIK. Bright objects that resembled keratocyte nuclei were manually counted by a masked observer. Cell densities were determined in anterior and posterior halves of the stromal flap, anterior and posterior halves of the 100-mum-thick layer immediately behind the ablation (retroablation layer), and the posterior third of the stroma. The region of stroma that was ablated (as measured 1 month after LASIK) was omitted from preoperative analysis. Cell densities after LASIK were compared (using1-factor repeated-measures analysis of variance) with densities in the corresponding layer of the normal preoperative stroma (which served as its own control).Main Outcome Measure: Corneal keratocyte density.Results: Before LASIK, keratocyte densities in the anterior and posterior stromal flap and the anterior retroablation layer were 34 818+/-5108 cells/mm(3) (mean +/- SD), 25 390+/-4045 cells/mm(3), and 21 328+/-2980 cells/mm(3), respectively, and densities in these layers decreased 14% to 20% at 1 month after LASIK (P