Disposable interference-free glucose biosensor based on an electropolymerised poly(pyrrole) permselective film
Disposable interference-free glucose biosensor based on an electropolymerised poly(pyrrole) permselective film
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DOI：
10.1016/s0003-2670(00)00998-3
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发表时间：
2000-09-07
影响因子：
6.2
通讯作者：
Zambonin, CG
中科院分区：
文献类型：
--
作者：
Quinto, M;Losito, I;Zambonin, CG
描述了用于测定血清中葡萄糖的微型一次性安培生物传感器。使用在平面陶瓷基底上产生的市售三电极系统作为生物传感器基底，并且通过电生成的过氧化聚（吡咯）膜（PPYox）来修饰工作Pt电极。在优化PPy层合成之后，研究了用于葡萄糖氧化酶（GOD）固定的两种不同方法：通过“全电化学”方法将酶包埋到电聚合膜中（PPYox/GOD）和通过与戊二醛/牛血清白蛋白共交联而在PPYox修饰电极上的凝胶包埋通过将50 μ l样品溶液滴到相关电极系统上进行的对葡萄糖的灵敏度的比较表明，两种传感器具有延伸至10 mM的线性范围，尽管Pt/PPYox/GOD-凝胶传感器更灵敏（168+/-15vs53 +/-7nAm/M），两种传感器显示出显著的抗干扰选择性，而且PPYox/GOD-gel传感器也具有良好的稳定性，可用于葡萄糖的流动注射分析，线性范围可达25 mM。（C）2000 Elsevier Science B. V.
Miniaturised disposable amperometric biosensors for glucose determination in serum are described. A commercially available three-electrode system created on a planar corundum ceramic base was used as biosensor substrate and the working Pt electrode was modified by an electrogenerated overoxidised poly(pyrrole) film (PPYox).After the optimisation of the PPy layer synthesis, two different approaches were investigated for glucose oxidase (GOD) immobilisation: enzyme entrapment into the electropolymerised film by an 'all-electrochemical' procedure (PPYox/GOD) and gel-entrapment over the PPYox modified electrode by co-crosslinking with glutaraldehyde/bovine serum albumin (PPYox/GOD-gel).A comparison of the sensitivities to glucose, performed by dropping 50 mu l of the sample solutions onto the relevant electrode systems, showed that both sensors have a linearity range extending up to 10 mM, though Pt/PPYox/GOD-gel sensor is more sensitive (168+/-15 versus 53+/-7 nAm/M), The two sensors showed remarkable anti-interferent selectivity, moreover the PPYox/GOD-gel sensor had also a good stability and could be used for flow injection analysis of glucose, with a linearity range extending up to 25 mM. (C) 2000 Elsevier Science B.V. All rights reserved.