Oxidation of reduced diphosphopyridine nucleotide.
Oxidation of reduced diphosphopyridine nucleotide.
复制标题
还原型二磷酸吡啶核苷酸的氧化。
DOI：
10.1016/s0021-9258(19)77327-x
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
L. Hellerman
中科院分区：
文献类型：
--
作者：
K. Schellenberg;L. Hellerman
结果图1描述了DPNH和新卟啉的反应。在405 mmol/L下测定初始过量使用的该电子受体的DPNH还原;通过加入GSH还原残留的氧化剂，然后测定形成的DPN。Ca.？c&tion-A OD（校正为3.0 ml）= 0.253.还原的新卟啉氧化物=（0.253 X 3.0）/1810= 4.2 X 10-* mmole。还原的新卟啉与初始DPNH的比率（2.05 X 10-3= 2.05.所形成的DPN = 1.81 × 10 m4毫摩尔（酶测定）;产率，所形成的DPN，88%。当反应在pH 8的焦磷酸盐缓冲液中进行时，该过程的化学计量保持不变，如下：
ResultsIn Fig. 1 is depicted the reaction of DPNH and new porphyrexide’(7). Reduction by DPNH of this electron acceptor, used in initial excess, wa,~ determined at 405 rnlr; the residual oxidant t, hen was reduced by the addition of GSH, followed by assay of the DPN formed. Ca.? c&tion-A OD (corrected to 3.0 ml.)= 0.253. New porphyrexide reduced=(0.253 X 3.0)/1810= 4.2 X lo-* mmole. Ratio, new porphyrexide reduced, to initial DPNH (2.05 X 10-3= 2.05. DPN formed= 1.81 X 10m4 mmole (enzymatic assay); yield, DPN formed, 88 per cent. The stoichiometry of the process, which remains the same when reaction is conducted at pH 8 in pyrophosphate buffer, is as follows: