VR1, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation
VR1, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation
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DOI：
10.1016/s0304-3959(02)00067-2
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发表时间：
2002-09-01
期刊：
影响因子：
7.4
通讯作者：
Noguchi, K
中科院分区：
文献类型：
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作者：
Fukuoka, T;Tokunaga, A;Noguchi, K
我们研究了外周组织中伤害性刺激的两种候选转导物，香草酸受体亚型1（VR 1）和P2 X（3），ATP的离子型P2 X受体的亚基，在L5脊神经结扎后的备用L4 DRG神经元中的表达，神经病理性疼痛模型。VR 1 mRNA在伤后1 ~ 28 d在DRG中小神经元中表达增加，这种表达的增加与热痛敏的发生和维持相一致。VR 1免疫反应（IR）神经元的增加在手术后第三天得到证实。相反，结扎后4周内P2 X（3）mRNA的表达没有变化，手术后3天观察到的P2 X（3）-ir神经元的百分比也没有变化。我们的数据表明，备用L4 DRG中VR 1的增加可能有助于在该神经性疼痛模型中观察到的夸大的热反应。结合之前关于其他神经性疼痛模型中备用背根节神经元中P2 X（3）表达增加的报道，各种神经性疼痛模型的表型变化和病理机制似乎存在差异。(C)2002年国际疼痛研究协会。由Elsevier Science B. V.出版，版权所有。
We investigated the expression of two candidate transducers of noxious stimuli in peripheral tissues, the vanilloid receptor subtype 1 (VR1) and the P2X(3), a subunit of the ionotropic P2X receptor for ATP, in spared L4 DRG neurons following L5 spinal nerve ligation, a neuropathic pain model. VR1 mRNA expression increased in the small- and medium-sized DRG neurons from the first to 28th day after injury, and this up-regulation corresponded well with the development and maintenance of thermal hyperalgesia of the hind paw. The increase in VR1-immunoreactive (ir) neurons was confirmed at the third day after surgery. In contrast, there was no change in expression of P2X(3) mRNA over 4 weeks after ligation, or in the percentage of P2X(3)-ir neurons observed 3 days after surgery. Our data suggests that increased VR1 in the spared L4 DRG may contribute to the exaggerated heat response observed in this neuropathic pain model. Taken together with the previous reports that P2X(3) expression increases in the spared DRG neurons in other neuropathic pain models, there appears to be differences in the phenotypic changes and pathomechanisms of the various neuropathic pain models. (C) 2002 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.