Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition
Release and reactive-oxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition
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DOI：
10.1093/pcp/pch027
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发表时间：
2004-02-01
影响因子：
4.9
通讯作者：
Yamamoto, Y
中科院分区：
文献类型：
--
作者：
Henmi, T;Miyao, M;Yamamoto, Y
在光抑制光照下，菠菜PSII膜释放出放氧复合物亚基OEC 33、24和18。释放的OEC 33和OEC 24随后在较小程度上被破坏，然后在SDS/尿素-PAGE中显示出模糊的条带。OEC 33一旦降解，就不能与PSII结合。清除剂和螯合剂对损伤的影响表明，在金属离子存在下，超氧化物产生的羟基自由基的损伤负责。这些结果表明，像D1蛋白的PSII反应中心复合物，OEC亚基遭受氧化损伤和营业额在光照下。
Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.