Low expression of long noncoding RNA CTC-297N7.9 predicts poor prognosis in patients with hepatocellular carcinoma
Low expression of long noncoding RNA CTC-297N7.9 predicts poor prognosis in patients with hepatocellular carcinoma
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长非编码 RNA CTC-297N7.9 的低表达预示肝细胞癌患者预后不良
DOI：
10.1002/cam4.2618
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发表时间：
2019-11-01
期刊：
影响因子：
4
通讯作者：
Shang, Changzhen
中科院分区：
文献类型：
--
作者：
Zhu, Sicong;Huang, Xuelian;Shang, Changzhen
据报道，长链非编码rna （lncRNAs）在多种恶性肿瘤的发生中发挥着重要作用。然而，lncRNA在肝细胞癌（HCC）中异常表达的临床意义尚不明确。方法首先，通过分析癌症基因组图谱（TCGA）的数据，检测HCC中差异表达的lncRNA CTC-297N7.9。其次，本中心采用聚合酶链反应（PCR）法检测CTC-297N7.9在4个肝癌细胞系和60对肝癌组织中的表达水平。再次，进行受试者工作特征（ROC）分析，评价CTC-297N7.9对HCC的诊断价值。我们还对TCGA和本中心HCC患者进行相关性和生存分析，以评估CTC-297N7.9的预测价值。最后，结合lncRNA表达及其他临床参数建立生存预后模型。结果CTC-297N7.9在肝癌细胞系和肝癌组织中表达下调。ROC曲线显示其对HCC的诊断价值。CTC-297N7.9的表达与血清甲胎蛋白（AFP）、肿瘤分期、肿瘤分化相关。生存分析表明，总生存期（OS）和无病生存期（DFS）均与CTC-297N7.9的表达呈正相关，特别是在无病毒性肝炎或肝硬化的患者中。Cox回归分析显示，CTC-297N7.9表达水平是HCC患者OS和DFS的独立预后因素。基于该模型，CTC-297N7.9与风险评分呈负相关，提示其是HCC的保护因子。结论CTC-297N7.9在HCC患者中低表达与预后不良相关，提示其可能是HCC的潜在预后标志物。
Background Long noncoding RNAs (lncRNAs) are reported to play important roles in tumorigenesis of various malignant tumors. However, the clinical significance of aberrant lncRNA expression in hepatocellular carcinoma (HCC) is still elusive. Methods Firstly, a differentially expressed lncRNA CTC-297N7.9 in HCC was detected by analyzing the data from The Cancer Genome Atlas (TCGA). Secondly, the expression level of CTC-297N7.9 was examined in four HCC cell lines and 60 pairs of HCC tissues by polymerase chain reaction (PCR) assay at our center. Thirdly, receiver operating characteristic (ROC) analysis was performed to evaluate the diagnostic value of CTC-297N7.9 for HCC. Correlation and survival analysis of HCC patients from the TCGA and our center were also carried out to assess the predictive value of CTC-297N7.9. Finally, survival prognostic models were established combining lncRNA expression and other clinical parameters. Results The expression of CTC-297N7.9 was downregulated in HCC cell lines and HCC tissues. ROC curve revealed its significant diagnostic value in HCC. CTC-297N7.9 expression correlated with serum alpha-fetal protein (AFP), tumor stage, and tumor differentiation. Survival analysis indicated that overall survival (OS) and disease-free survival (DFS) are all positively associated with CTC-297N7.9 expression, especially in patients without viral hepatitis or cirrhosis. Cox regression analysis showed that CTC-297N7.9 expression level is an independent prognostic factor for both OS and DFS in HCC patients. Based on the model, CTC-297N7.9 was observed to be negatively correlated to risk score, indicating its role as a protective factor for HCC. Conclusion Our study demonstrated that the low expression of CTC-297N7.9 is associated with poor prognosis in HCC patients, suggesting its possible role as a potential prognostic marker for HCC.