The kinetochore is part of the metaphase chromosome scaffold.
The kinetochore is part of the metaphase chromosome scaffold.
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DOI：
10.1083/jcb.98.1.352
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发表时间：
1984-01
期刊：
影响因子：
--
通讯作者：
Rothfield N
中科院分区：
文献类型：
--
作者：
Earnshaw WC;Halligan N;Cooke C;Rothfield N
我们使用硬皮病CREST综合征（钙质沉着症、雷诺现象、食道运动障碍、硬指症、毛细血管扩张）患者的抗血清，证明中期染色体中有着丝点的抗原成分也存在于大量消化DNA和提取大量染色体蛋白后分离的非组蛋白染色体支架中。免疫荧光显微镜显示，12例硬皮病CREST患者的血清中含有循环抗着丝点抗体，在免疫印迹分析中识别Mr 77000蛋白（CREST-77）。12种血清中有9种也能识别Mr 110,000抗原（CREST-110）。这些蛋白存在于分离的染色体和非组蛋白支架中，通过两种不同的方法从它们中获得。5例抗着丝粒阴性（免疫荧光）的硬皮病CREST患者的血清在免疫印迹中不与任何蛋白结合。这些数据表明，CREST-77（也可能是CREST-110）是人类着丝点的一个组成部分，着丝点是有丝分裂染色体支架的一个组成部分。
We used antisera from patients with the CREST syndrome of scleroderma (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) to show that an antigenic component of the kinetochore present in metaphase chromosomes is also present in nonhistone chromosome scaffolds isolated following extensive digestion of the DNA and extraction of the bulk of chromosomal protein. All sera from 12 scleroderma CREST patients previously shown by immunofluorescence microscopy to have circulating antikinetochore antibodies recognise a protein of Mr 77,000 (CREST-77) in an immunoblotting assay. 9 of the 12 sera also recognise an antigen of Mr 110,000 (CREST-110). These proteins are present in isolated chromosomes and nonhistone scaffolds derived from them by two different procedures. Sera of five scleroderma CREST patients who are antikinetochore negative (by immunofluorescence) bind to neither protein in immunoblots. These data suggest that CREST-77 (and possibly CREST-110) is a component of the human kinetochore, and that the kinetochore is an integral part of the mitotic chromosome scaffolding.