Current methodologies for the study of pineal morphophysiology.
Current methodologies for the study of pineal morphophysiology.
复制标题
当前松果体形态生理学研究的方法。
DOI：
10.1111/j.1600-079x.1994.tb00090.x
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发表时间：
1994
影响因子：
10.3
通讯作者：
Welsh,MG
中科院分区：
文献类型：
--
作者：
Welsh,MG
威尔士MG松果体形态生理学研究的现代方法学。1994; 16：113- 120.摘要光镜和电子显微镜，使用或不使用免疫组织化学技术，一直是研究松果体复合体的首选仪器，甚至到最近的时间。其他形态学技术已成为在过去的十年中，如果适用于当前的问题，松果体形态生理学，可以大大增加我们对这个复杂系统的理解。这些技术包括共聚焦扫描激光显微镜（结合其他技术，包括免疫组织化学和三维重建），组织培养方法，碳菁染料（即。例如，DiI）、原位杂交和显微注射方法学的应用。这表明，这些技术将是必要的形态生理学家不仅与分子生物学家和生物化学家谁研究松果体复合体合作，但证实我们的同事的分子和生化结果。
Welsh MG. Current methodologies for the study of pineal morphophysiology. J Pineal Res 1994; 16: 113–120.AbstractLight and electron microscopes, with or without the use of immunohistochemical techniques, have been the instruments of choice for study of the pineal complex even up to recent times. Other morphological technologies have become available during the past decade that, if applied to current questions concerning pineal morphophysiology, could add considerably to our understanding of this complex system. Those technologies discussed include confocal scanning laser microscopy (in conjunction with other techniques including immunohistochemistry and three‐dimensional reconstruction), tissue culture methodologies, carbocyanine dyes (i. e., DiI), in situ hybridization, and application of microinjection methodologies. It is suggested that these technologies will be necessary for morphophysiolo‐gists to not only collaborate with molecular biologists and biochemists who study the pineal complex, but to corroborate the molecular and biochemical results of our colleagues.