Real-time and long-time in vivo imaging in the shortwave infrared window of perforator vessels for more precise evaluation of flap perfusion
Real-time and long-time in vivo imaging in the shortwave infrared window of perforator vessels for more precise evaluation of flap perfusion
复制标题
穿支血管短波红外窗口实时长时间体内成像，更精确地评估皮瓣灌注
DOI：
10.1016/j.biomaterials.2016.06.043
复制
发表时间：
2016-10-01
期刊：
影响因子：
14
通讯作者：
Zhang, Wenjie
中科院分区：
文献类型：
--
作者：
Feng, Shaoqing;Chen, Jun;Zhang, Wenjie
对皮瓣灌注进行有效的实时、长时间的活体成像需要明亮、稳定的显像剂，其辐射能有效穿透活组织而不发生光漂白。与目前的标准成像剂——术中吲哚菁绿（ICG）相比，量子点（QDs）是一种更有吸引力的替代方案，因为它具有优异的光学特性，包括广泛的发射光谱和抗光漂白的稳定性。最近的研究证实，1000 ~ 2300 nm的短波红外窗口（SWIR）由于其极低的组织吸收和自身荧光，是体内成像最敏感的光谱范围。在这里，我们首次报道了一种新的皮瓣灌注评估方法，该方法使用硫化铅（PbS）量子点提供实时和长时间的体内成像。我们的研究结果表明，PbS量子点作为显像剂，可以持续提高体内高分辨率图像的稳定性，从而促进皮瓣灌注的精确评估。综上所述，与目前的成像报告相比，SWIR qd具有高光稳定性和深组织穿透性，使其成为更精确评估皮瓣灌注的活体显像剂。(C) 2016 Elsevier Ltd.版权所有。
Effective real-time and long-time in vivo imaging for flap perfusion requires bright and stable imaging agents whose emissions can effectively penetrate live tissues without photobleaching. Compared to the standard imaging agent today - intraoperative indocyanine green (ICG), quantum dots (QDs) is a more attractive alternative due to its excellent optical properties including broad emission spectrum and stability against photobleaching. Recent studies have confirmed that the shortwave infrared window (SWIR) between 1000 and 2300 nm is the most sensitive spectral range for in vivo imaging due to its extremely low tissue absorption and autofluorescence. Here, we, for the first time, report a novel approach of flap perfusion assessment that provides real-time and long-time in vivo imaging using lead sulfide (PbS) QDs. Our results show that PbS QDs, as an imaging agent, can improve the stability of in vivo high-resolution images in a sustained manner, thus facilitating the precise evaluation of flap perfusion. In summary, compared to current imaging reporters, SWIR QDs have high photostability and deep tissue penetration, which makes them as promising in vivo imaging agents for more precise evaluation of flap perfusion. (C) 2016 Elsevier Ltd. All rights reserved.