Proteomic identification of human sperm proteins
Proteomic identification of human sperm proteins
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DOI：
10.1002/pmic.200600094
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发表时间：
2006-08-01
期刊：
影响因子：
3.4
通讯作者：
Oliva, Rafael
中科院分区：
文献类型：
--
作者：
Martinez-Heredia, Juan;Estanyol, Josep Maria;Oliva, Rafael
传统的1-DE在过去已经提供了关于主要精子蛋白质的丰富信息。然而，到目前为止，利用目前蛋白质组学工具的潜力来鉴定和研究存在于人类精子中的其他尚未鉴定的重要蛋白质的报告相对较少。在本工作中，从人类正常精子中提取的蛋白质的双向凝胶电泳法获得了1000多个斑点的分辨率。随后从145个点的凝胶中切除，并进行MALDI-TOF MS分析，从而鉴定出98种不同的蛋白质。这些蛋白质的功能包括能量产生(23%)、转录、蛋白质合成、运输、折叠和周转(23%)、细胞周期、细胞凋亡和氧化应激(10%)、信号转导(8%)、细胞骨架、鞭毛和细胞运动(10%)、细胞识别(7%)、代谢(6%)和未知功能(11%)。在已鉴定的蛋白质中，多达23%以前从未被描述为在人类精子中表达。目前的数据为确定这些蛋白质的功能提供了重要线索，并为进行更多的实验打开了可能性。
Conventional 1-DE has in the past provided a wealth of information concerning the major sperm proteins. However, so far there are relatively few reports exploiting the potential of the present proteomic tools to identify and to study additional yet-unidentified important proteins present in human spermatozoa. In the present work, 2-DE of proteins extracted from human normozoospermic spermatozoa led to the resolution of over 1000 spots. Subsequent excision from the gels of 145 spots and MALDI-TOF MS analysis allowed the identification of 98 different proteins. The function of these proteins turned out to be energy production (23%), transcription, protein synthesis, transport, folding and turnover (23%), cell cycle, apoptosis and oxidative stress (10%), signal transduction (8%), cytoskeleton, flagella and cell movement (10%), cell recognition (7%), metabolism (6%) and unknown function (11%). As many as 23% of the proteins identified have not been previously described as being expressed in human spermatozoa. The present data provide an important clue towards determining the function of these proteins and opens up the possibility to perform additional experiments.