Predictive serum markers for unexplained infertility in child-bearing aged women
Predictive serum markers for unexplained infertility in child-bearing aged women
复制标题
育龄妇女不明原因不孕的预测血清标志物
DOI：
10.1111/aji.13194
复制
发表时间：
2020
影响因子：
3.6
通讯作者：
Gu Jiang
中科院分区：
文献类型：
--
作者：
Qu Ting;Yan Meiling;Shen Wen-Jun;Li Lingxiao;Zhu Ping;Li Zhouxuan;Huang Jihua;Han Tingting;Hu Wenhui;Zhou Ruinian;Li Penghao;Xu Lan;Huang Tianhua;Zhong Ying;Gu Jiang
原因不明的不孕症（UI）约占所有不孕症的25%-40%，是育龄妇女成功怀孕的巨大障碍。然而，到目前为止，还没有可靠的方法来预测这种情况的高精度，从而阻碍了早期管理这种condition.Method研究我们的前瞻性研究包括84育龄妇女的临床诊断UI。44名匹配的健康生育（HF）女性作为对照。我们利用蛋白质芯片技术检测了25种可能与UI发病机制和分子通路相关的激素和细胞因子的表达谱。将样本按7：3随机分层为训练集和测试集。我们使用的SMOTEboost模型与10血清蛋白在临床验证study中，以确定UI cases.ResultsThe预测的受试者工作特征曲线下面积（AUC）为0.788与24血清蛋白功能。在临床验证研究中对UI病例进行分类的前10种重要血清蛋白模型的AUC预测性能为0.809。三个最显着的差异表达蛋白质（DEPs）是催乳素，单核细胞趋化蛋白-1（MCP-1），和leptin.ConclusionExamination血清蛋白质谱的变化可能有助于确定育龄妇女在UI的风险。这将使早期发现和促进临床战略的发展，以治疗UI和指导他们的计划生育。它也可能提供线索的情况下，测试对象的发病机制。
ProblemUnexplained infertility (UI) represents about 25%‐40% of all infertility and is a formidable obstacle for successful pregnancy for child‐bearing aged women. However, up to now, there is no reliable method to predict this condition with high accuracy, thereby hindering early management of this condition.Method of studyOur prospective study consists of 84 child‐bearing aged women that were clinically diagnosed UI. Forty‐four matched healthy fertility (HF) women were served as controls. We examined the profiles of 25 hormones and cytokines that were likely related to pathogeneses and molecular pathways involved in UI with the technique of protein array. The samples were randomly stratified 7:3 into a training set and a testing set. We used the SMOTEboost model with 10 serum proteins in a clinical verification study to identify UI cases.ResultsThe predictor had an area under the receiver operating characteristic curve (AUC) of 0.788 with 24 serum protein features. The predictive performance in terms of AUC of the model with the top 10 important serum proteins in the clinical verification study to classify UI cases was 0.809. Three most significantly differentially expressed proteins (DEPs) were prolactin, monocyte chemotactic protein‐1 (MCP‐1), and leptin.ConclusionExamination of serum‐based protein profile changes could help to identify child‐bearing aged women at risk of UI. This would enable early detection and facilitate development of clinical strategies to treat UI and guide their planned parenthood. It may also give clues to pathogeneses of the condition of test subjects.