A Miniature Graphene-based Biosensor for Intracellular Glucose Measurements
A Miniature Graphene-based Biosensor for Intracellular Glucose Measurements
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DOI：
10.1016/j.electacta.2015.06.035
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发表时间：
2015-08-20
影响因子：
6.6
通讯作者：
Stralfors, Peter
中科院分区：
文献类型：
--
作者：
ul Hasan, Kamran;Asif, Muhammad H.;Stralfors, Peter
我们报道了一种用于测量细胞内葡萄糖浓度的小而简单的石墨烯电位传感器。将涂有石墨烯并随后固定有葡萄糖氧化酶（GOD）的精细硼硅酸盐玻璃毛细管插入单个人类细胞的细胞内环境中。石墨烯边缘平面上的官能团有助于与GOD酶的游离胺末端的连接，导致更好的固定。该传感器表现出葡萄糖依赖性的电化学电位对Ag/AgCl参考微电极，这是线性的整个浓度范围内的利益（10 - 1000 μ M）。我们的石墨烯基传感器测量的人体脂肪细胞中的葡萄糖浓度与核磁共振（NMR）光谱是很好的协议。(C)2015爱思唯尔有限公司版权所有。
We report on a small and simple graphene-based potentiometric sensor for the measurement of intracellular glucose concentration. A fine borosilicate glass capillary coated with graphene and subsequently immobilized with glucose oxidase (GOD) enzyme is inserted into the intracellular environment of a single human cell. The functional groups on the edge plane of graphene assist the attachment with the free amine terminals of GOD enzyme, resulting in a better immobilization. The sensor exhibits a glucose-dependent electrochemical potential against an Ag/AgCl reference microelectrode which is linear across the whole concentration range of interest (10 - 1000 mu M). Glucose concentration in human fat cell measured by our graphene-based sensor is in good agreement with nuclear magnetic resonance (NMR) spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved.