Genetic Diversity and Pathogenic Variation of the Rice False Smut Pathogen Ustilaginoidea virens from Different Rice Cultivars
Genetic Diversity and Pathogenic Variation of the Rice False Smut Pathogen Ustilaginoidea virens from Different Rice Cultivars
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DOI：
10.1094/phyto-03-22-0099-r
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发表时间：
2022-11-08
期刊：
影响因子：
3.2
通讯作者：
Fang, Anfei
中科院分区：
文献类型：
--
作者：
Bai, Zhenxu;Qin, Yubao;Fang, Anfei
稻曲病是由稻曲病菌（Ustilaginoidea virens）引起的世界性水稻病害之一。了解美国的遗传多样性。virens对于有效的疾病控制和抗病育种是必不可少的。然而，对U.来自不同水稻品种的病毒。本研究调查了乌骨鸡的遗传多样性和致病性变异。从浙江省10个水稻品种中分离到的virens菌株。共鉴定出260个多态位点和27个单倍型，其中hap_4为最常见的单倍型，有41个菌株。系统发育分析表明，所有菌株可分为4个遗传群。第I类最大，有98个菌株，主要分布在8个品种群体，而90%的分离物收集从长香品种聚在第IV类。在27个群体间的成对比较中，F-ST值表现出显著的遗传分化，占总遗传变异的23.21%。CX群体的分离物的遗传组成与其他9个群体的分离物的遗传组成不同，并且在少数分离物中发现了遗传重组。最后，27个单倍型代表菌株的致病性表现出高度的变异，从遗传亚群I的菌株可能是更强的毒性比那些从遗传亚群II和III。总的来说，这些研究结果表明，水稻品种的差异在美国的遗传变异中起着重要的作用。绿色的
Rice false smut, caused by Ustilaginoidea virens, has become one of the most devastating grain diseases of rice worldwide. Understanding the genetic diversity of U. virens is essential for efficient disease control and breeding for disease resistance. However, little is known about the genetic variation of U. virens from different rice cultivars. We investigated the genetic diversity and pathogenic variation of U. virens isolates from 10 rice cultivars in Zhejiang, China. A total of 260 polymorphic loci and 27 haplotypes were identified based on the 2,137-bp combined DNA fragments of all individuals; hap_4 was the most common haplotype, represented by 41 isolates. Phylogeny indicated that all isolates were divided into four genetic groups. Group I was the largest, with 98 isolates, distributed mainly in eight cultivar populations, whereas 90% of the isolates collected from a Changxiang cultivar were clustered in Group IV. Furthermore, the pairwise F-ST values exhibited significant genetic differentiation in 27 of the pairwise comparisons between populations, accounting for 23.21% of the total genetic variation. The genetic composition of the isolates of the CX population was distinguishable from that of the other nine populations, and genetic recombination was found in a few isolates. Finally, 27 haplotype representative isolates showed high variation in pathogenicity, and the isolates from the genetic subpopulation I were likely to be more virulent than those from genetic subpopulations II and III. Collectively, these findings suggest that differences in rice cultivars play an important role in the genetic variation of U. virens.