Fluorescence Anisotropy in a Protein: DNA System Undergoing Inducible Assembly
Fluorescence Anisotropy in a Protein: DNA System Undergoing Inducible Assembly
复制标题
蛋白质中的荧光各向异性：进行诱导组装的 DNA 系统
DOI：
10.1016/j.bpj.2012.11.2200
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发表时间：
2013
影响因子：
3.4
通讯作者：
Gholami Z
中科院分区：
文献类型：
--
作者：
Gholami Z
Brookhaven STEM在40 keV下工作，探针尺寸为0.3 nm，通过聚焦来自冷场发射源的光束获得。试样保持在160 ℃以减少质量损失.将样本快速冷冻，冻干过夜并在真空下转移到显微镜。量子效率探测器测量通过样品的每个电子：未散射（亮场），以小角度散射（SA）或以大角度散射（LA，暗场）。LA信号用于质量测量，因为它没有相干散射效应（散焦相位对比，晶体上的衍射对比）。离线分析屏蔽颗粒以确定背景，找到细丝段并测量信号减去背景，转换为单位长度质量。所有测量值都存储在数据库中，并进行统计分析。http：//www. bnl。政府/生物学/干/
The Brookhaven STEM operates at 40 keV with a probe size of 0.3 nm obtained by focusing a beam from a cold field emission source. The specimen is maintained at À160 C to reduce mass loss. Specimens are fast frozen, freeze dried overnight and transferred under vacuum to the microscope. Quantum efficiency detectors measure every electron passing through the specimen as: unscattered (bright field), scattered at small angles (SA) or scattered at large angles (LA, dark field). The LA signal is used for mass measurements since it is free of coherent scattering effects (defocus phase contrast, diffraction contrast on crystals). Off-line analysis masks particles to determine background, finds filament segments and measures signal minus background, converting to mass-per-unit-length. All measurements are stored in a database with statistical analysis. http://www. bnl. gov/biology/stem/