CHANGES IN POLYADENYLATION OF LACTATE DEHYDROGENASE-X MESSENGER RNA DURING SPERMATOGENESIS IN MICE
CHANGES IN POLYADENYLATION OF LACTATE DEHYDROGENASE-X MESSENGER RNA DURING SPERMATOGENESIS IN MICE
复制标题
DOI：
10.1002/mrd.1080010106
复制
发表时间：
1988-01-01
影响因子：
2.5
通讯作者：
TONE S
中科院分区：
文献类型：
--
作者：
FUJIMOTO H;ERICKSON R P;TONE S
用RBA：cDNA原位杂交法和北方分析法检测了小鼠睾丸乳酸脱氢酶（LDH-X）mRNA的表达。银颗粒积累在生精小管周边第二层内的细胞中，证实了先前的研究结果，即LDH-X mRNA首先出现在精母细胞中，并继续积累，直到精子细胞晚期。北方分析表明，减数分裂和减数分裂后的细胞分别含有1.2和1.3 kb的杂交mRNA类。RNase H消化oligo（dT）-杂交的RNA和poly（U）-Sepharose柱层析，用甲酰胺差示洗脱，揭示了两类mRNA大小的差异是由于LDH-X mRNA的poly（A）尾长度。当检查LDH-X mRNA在多聚核糖体梯度中的分布时，两种mRNA都与多聚核糖体部分相关。这些结果表明，小鼠精子发生过程中LDH-X mRNA多聚腺苷酸化的变化与减数分裂有关。他们提出了这样的可能性，即LDH-X mRNA在减数分裂后细胞中的稳定积累是通过增加长度的poly（A）尾来增强的。
The expression of the mRNA for mouse testicular lactate dehydrogenase (LDH-X) was examined by RBA:cDNA hybridization in situ in the testis and by Northern analyses of meiotic and postmeiotic spermatogenic cell populations. Silver grains accumulated in cells inside the second layer from the periphery of the seminiferous tubule, confirming previous findings that LDH-X mRNA first appears in the spermatocyte and continues to accumulate until the late spermatid stage. Northern analyses showed that meiotic and post-meiotic cells contained 1.2 and 1.3 kb classes of hybridizing mRNA, respectively. RNase H digestion of oligo(dT)-hybridized RNA and poly(U)-Sepharose column chromatography with differential elution by formamide revealed that the difference in size of the two classes of mRNAs was due to the poly(A) tail length of the LDH-X mRNA. When the distribution of the LDH-X mRNA was examined across polysome gradients, both mRNAs were partially associated with polysomes. These results suggest that the changes in the polyadenylation of LDH-X mRNA were associated with the meiotic division during spermatogenesis in the mouse. They raise the possibility that the stable accumulation of the LDH-X mRNAs in the postmeiotic cells is enhanced by poly(A) tails of increased length.