DISTRIBUTION AND FUNCTIONAL-PROPERTIES OF 5-HT3, RECEPTORS IN THE RAT HIPPOCAMPAL DENTATE GYRUS - A PATCH-CLAMP STUDY
DISTRIBUTION AND FUNCTIONAL-PROPERTIES OF 5-HT3, RECEPTORS IN THE RAT HIPPOCAMPAL DENTATE GYRUS - A PATCH-CLAMP STUDY
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DOI：
10.1152/jn.1994.71.5.1935
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发表时间：
1994-05-01
影响因子：
2.5
通讯作者：
KAWA, K
中科院分区：
文献类型：
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作者：
KAWA, K
1.在Nomarski显微镜下，可在大鼠海马片齿状回内观察到两种不同类型的神经元，即主要兴奋性神经元(颗粒细胞)和局部抑制性神经元(篮状细胞)；细胞内荧光法黄染证实了神经元的存在。在篮状细胞和颗粒细胞中，全细胞电流记录显示，在保持电位为-63 mV的对称氯离子条件下，自发突触电流范围为-lt；10pA到>；200pA。这些电流被10u M荷包牡丹碱阻断，表明它们是由GABA能传入的自发激活引起的(这在两种类型的神经元中都有形态上的描述)。将5-羟色胺(2-50微米)集中应用于篮筐细胞，在电流钳下，诱发出叠加在基线膜去极化上的一系列动作电位。在电压钳条件下，5-羟色胺在-63 mV的保持电位下诱发内向电流(电流与所研究的篮子细胞的90%相似)。内流伴随着大量持续时间较短的小内流(
1. In dentate gyrus of rat hippocampal slices two distinct types of neurons, principal excitatory neurons (granule cells) and local inhibitory neurons (basket cells), could be identified under Nomarski microscopy; I investigated the actions of serotonin using the whole-cell patch-clamp technique. The identification of the neurons was later confirmed by intracellular staining with Lucifer yellow.2. In both basket cells and granule cells, whole-cell current recordings revealed spontaneous synaptic currents ranging from < 10 pA to > 200 pA in symmetrical Cl- conditions at a holding potential of -63 mV. These currents were blocked by 10 mu M bicuculline, indicating that they resulted from the spontaneous activation of GABAergic inputs (which had been morphologically described in both types of neurons).3. By focal application of serotonin (2-50 mu M) to basket cells under current clamp I evoked a train of action potentials superimposed on a baseline membrane depolarization. Under voltage-clamp conditions serotonin evoked an inward current at a holding potential of -63 mV (currents were detectable in similar to 90% of basket cells studied). The inward current was accompanied by a multitude of small inward currents of short duration (