Mass spectrometry imaging of small molecules in biological tissues using graphene oxide as a matrix
Mass spectrometry imaging of small molecules in biological tissues using graphene oxide as a matrix
复制标题
使用氧化石墨烯作为基质对生物组织中的小分子进行质谱成像
DOI：
10.1016/j.aca.2017.01.043
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发表时间：
2017
影响因子：
6.2
通讯作者：
Li Zhili
中科院分区：
文献类型：
--
作者：
Zhou Dan;Guo Shuai;Zhang Mo;Liu Yujie;Chen Tianjing;Li Zhili
随着基质辅助激光解吸/电离质谱成像（MALDI-MSI）技术的发展，大范围组织切片的分子检测已成为可能，但由于灵敏度和基质干扰的限制，小分子分析还远未完全实现。在本文中，氧化石墨烯（GO）用作MALDI基质以负离子模式对组织中的小分子进行成像。最后，在小鼠脑组织切片中检测到212种分子，包括190种脂类和22种低分子量代谢物，在不受基质离子/团簇干扰的情况下进行了空间可视化，其中69种脂类的结构通过原位串联质谱得到了确认。GO矩阵的进一步应用可以揭示源自小鼠乳腺癌组织的存活和坏死肿瘤区域中的不同空间分子特征。此外，GO作为MALDI基质，相对于N-（1-萘基）乙二胺二盐酸盐和9-氨基吖啶，在脂质的MSI中表现出更好的性能。
With the development of matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI), molecular interrogation of tissue sections over a wide mass range has become feasible, but small molecule analysis is still far from being fully reached due to the limited sensitivity and matrix interference. Herein, graphene oxide (GO) is used as a MALDI matrix to image small molecules in tissues in negative ion mode. Finally, 212 of molecules including 190 of lipids and 22 of low molecular weight metabolites were detected and spatially visualized in mouse brain tissue sections without the interference of matrix ions/clusters, and the structures of 69 of the lipids were confirmed by usingin situtandem mass spectrometry. A further application of GO matrix could reveal distinct spatio-molecular signatures in viable and necrotic tumor regions derived from a mouse breast cancer tissue. In addition, GO as a MALDI matrix has exhibited a better performance in MSI of lipids relative to N-(1-naphthyl) ethylenediamine dihydrochloride and 9-aminoacridine.