High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori
High-efficient expression of porcine IL-2 with recombinant baculovirus infected silkworm,Bombyx mori
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重组杆状病毒感染家蚕高效表达猪IL-2
DOI：
10.1007/bf02931887
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发表时间：
2000
影响因子：
3.2
通讯作者：
M. Miyazawa
中科院分区：
文献类型：
--
作者：
S. Inumaru;T. Kokuho;T. Yada;M. Kiuchi;M. Miyazawa
本研究利用苜蓿银纹夜蛾核型多角体病毒（Autographa californicanuclear polyhedrosis virus，AcNPV）-细胞培养系统和杂交核型多角体病毒（Hybrid nuclear polyhedrosis virus，HyNPV）-家蚕幼虫系统，分别在体外和体内杆状病毒表达系统中表达具有生物活性的猪白细胞介素-2（porcine Interleukin-2，poIL-2）。重组poIL-2（rpoIL-2）在幼虫血淋巴中的浓度为1 ~ 3 mg/mL，约为细胞培养体系的7 ~ 20倍。该表达效率的水平与转基因家畜在牛奶中分泌产物的水平相等。
Biologically active porcine Interleukin-2(poIL-2) was produced fromin vitroandin vivobaculovirus expression systems, namely theAutographa californicanuclear polyhedrosis virus (AcNPV)-cell culture system and the Hybrid nuclear polyhedrosis virus (HyNPV)— silkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.