Intrinsic cell permeability of the GAGA zinc finger protein into HeLa
Intrinsic cell permeability of the GAGA zinc finger protein into HeLa
复制标题
GAGA 锌指蛋白对 HeLa 的内在细胞渗透性
DOI：
10.1016/j.bbrc.2015.07.060
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发表时间：
2015
影响因子：
3.1
通讯作者：
Yukio Sugiura
中科院分区：
文献类型：
--
作者：
Shigeru Neg*i;Yuka terada;Misato Suzuyama;Makoto Matsumoto;Akino Honbo;Yoko Amagase;Yumiko Mizukawa;Akiko Kiriyama;Kastumi Iga;Tetsuro Urushidani;Yukio Sugiura
我们检测了从果蝇黑胃转录因子中获得的GAGA锌指的内在细胞通透性，并利用共聚焦显微镜和流式细胞术分析了其细胞摄取机制。用cy5标记的GAGA肽（含荧光发色团）处理HeLa细胞，用共聚焦显微镜检测荧光标记肽的荧光信号。结果清楚地表明，GAGA肽对HeLa细胞具有内在的细胞通透性。根据流式细胞仪分析结果和GAGA肽的理论净正电荷，预测GAGA肽的细胞摄取效率取决于GAGA肽的净正电荷以及Arg残基与Lys残基的阳离子组成比。
We examined the intrinsic cell permeability of a GAGA zinc finger obtained from theDrosophila melanogastertranscription factor and analyzed its mechanism of cellular uptake using confocal microscopy and flow cytometry. HeLa cells were treated with the Cy5-labeld GAGA peptides (containing a fluorescent chromophore) to detect fluorescence signals from the fluorescent labeling peptides by confocal microscopy. The results clearly indicated that GAGA peptides possess intrinsic cell permeability for HeLa cells. Based on the results of the flow cytometry analysis and the theoretical net positive charge of the GAGA peptides, the efficiency of cellular uptake of the GAGA peptides was predicted to depend on the net positive charge of the GAGA peptide as well as the cationic component ratio of Arg residues to Lys residues.