Long-term measurement of arterial blood pressure in conscious mice
Long-term measurement of arterial blood pressure in conscious mice
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DOI：
10.1152/ajpregu.1998.274.2.r564
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发表时间：
1998-02-01
影响因子：
2.8
通讯作者：
Mattson, DL
中科院分区：
文献类型：
--
作者：
Mattson, DL
本研究描述了一种技术，直接每天测量动脉血压，动脉血采样，并连续静脉输注自由活动，清醒，瑞士韦伯斯特小鼠。将导管长期植入股动脉和静脉中，皮下隧道化，在颈部后部的轻质拴系弹簧中外置，并连接到笼顶部的旋转装置。时间控制实验（n = 8）证明了平均动脉压（MAP，116 +/- 1 mmHg）和心率（HR，627 +/- 21次/min）在导管植入后长达35天的稳定值。进一步观察到，与自由活动条件下观察到的值相比，限制小鼠（n = 7）使MAP增加10 +/- 3 mmHg，HR增加78 +/- 8次/min。为了证明静脉导管的长期使用，静脉内输注N-G-硝基-L-精氨酸甲酯（L-NAME，8.6 mg.kg（-1）.day（-1），n = 6）5天显著地将MAP从117 +/-4增加到131 +/-4 mmHg而不改变HR。在最后一组小鼠（n = 5）中，口服L-精氨酸（2%在饮用水中）将血浆精氨酸浓度从90 +/-7增加到131 +/-17 μ M并防止L-NAME高血压。这些实验说明了在清醒小鼠中进行长期静脉输注、直接动脉血压测量和动脉采血的可行性。
This study describes a technique for the direct daily measurement of arterial blood pressure, sampling of arterial blood, and continuous intravenous infusion in free-moving, conscious, Swiss-Webster mice. Catheters were chronically implanted in the femoral artery and vein, tunneled subcutaneously, exteriorized at the back of the neck in a lightweight tethering spring, and attached to a swivel device at the top of the cage. Time-control experiments (n = 8) demonstrated stable values of mean arterial pressure (MAP, 116 +/- 1 mmHg) and heart rate (HR, 627 +/- 21 beats/min) for up to 35 days after catheter implantation. It was further observed that restraining mice (n = 7) increased MAP by 10 +/- 3 mmHg and HR by 78 +/- 8 beats/min from the values observed under free-moving conditions. To demonstrate the chronic use of the venous catheter, intravenous infusion of N-G-nitro-L-arginine methyl ester (L-NAME, 8.6 mg.kg(-1).day(-1), n = 6) for 5 days significantly increased MAP from 117 +/- 4 to 131 +/- 4 mmHg without altering HR. In a final group of mice (n = 5), oral L-arginine (2% in drinking water) increased plasma arginine concentration from 90 +/- 7 to 131 +/- 17 mu M and prevented L-NAME hypertension. These experiments illustrate the feasibility of long-term intravenous infusion, direct arterial blood pressure measurements, and arterial blood sampling in conscious mice.