Assessment of graphene oxide toxicity on the growth and nutrient levels of white clover (Trifolium repens L.).
Assessment of graphene oxide toxicity on the growth and nutrient levels of white clover (Trifolium repens L.).
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DOI：
10.1016/j.ecoenv.2022.113399
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发表时间：
2022-03
影响因子：
6.8
通讯作者：
Shulan Zhao;Xiangui Zhu;Mengdi Mou;Ziyuan Wang;L. Duo
中科院分区：
文献类型：
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作者：
Shulan Zhao;Xiangui Zhu;Mengdi Mou;Ziyuan Wang;L. Duo
碳纳米材料是一种新型的工程纳米材料，得到了广泛的应用。它们对土壤基质中的陆地植物的毒性作用需要仔细调查。在本研究中，白三叶草(Trifolium repensL.)在盆栽土壤中分别添加0.2%、0.4%和0.6%的氧化石墨烯(GO)，研究了GO对白三叶生长和养分吸收的影响。GO暴露对幼苗生长、光合作用参数和地上部养分吸收均有不利影响，且随着浓度的增加和暴露时间的延长，影响更为显著。与对照相比，0.6%浓度的GO处理100d后株高、茎叶干重、总叶绿素含量和净光合速率分别下降43.7%、45.7%、43.4%、32%和85.7%，N、K、Cu、Zn、Fe、Mo、B、Si含量分别下降19.5%、20.1%、12.6%、25.0%、12.9%、26.0%、18.9%和23.0%。此外，GO还增加了细胞的电解质外渗率、脂质过氧化作用、活性氧含量和抗氧化酶活性，尤其是在高剂量和长时间暴露时。这些结果表明，GO可以通过氧化胁迫、光合作用抑制和营养失衡来抑制植物的生长。
Carbon nanomaterials (CNMs) are novel engineered nanomaterials and have been used widely. Their toxic effects on terrestrial plants in soil matrix require careful investigation. In this study, white clover (Trifolium repensL.) was grown in a potted soil with graphene oxide (GO) at levels of 0.2%, 0.4% and 0.6% and the effects of GO on the growth and nutrient uptake of white clover were evaluated after 50 and 100 days of exposure. GO exposure showed adverse effects on seedling growth, photosynthetic parameters and nutrient uptake in shoots, and the effect was more significant with increasing concentration and exposure time. Compared with the control, GO at the highest level of 0.6% decreased plant height, leaf and stem dry weights, total chlorophyll content and net photosynthetic rate by 43.7%, 45.7%, 43.4%, 32% and 85.7%, respectively, after 100 d of exposure, and N, K, Cu, Zn, Fe, Mo, B, Si contents decreased by 19.5%, 20.1%, 12.6%, 25.0%, 12.9%, 26.0%, 18.9%, 23.0%, respectively. Furthermore, the electrolyte leakage, lipid peroxidation, reactive oxygen species, antioxidant enzyme activities were all increased by GO, especially at high dose and long exposure. These results indicate that GO can suppress plant growth by oxidative stress, photosynthesis inhibition, and nutrient imbalance.