Keratinization of the adult male foreskin and implications for male circumcision.
Keratinization of the adult male foreskin and implications for male circumcision.
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DOI：
10.1097/qad.0b013e3283367779
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发表时间：
2010-03-27
期刊：
影响因子：
--
通讯作者：
Hope TJ
中科院分区：
文献类型：
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作者：
Dinh MH;McRaven MD;Kelley Z;Penugonda S;Hope TJ
较薄的角化内包皮会导致 HIV-1 易感性增加的理论基于相对较少的已发表数据。我们试图量化内包皮和外包皮的角蛋白厚度，以确定该假设的合理性。我们对 16 名成年男性包皮的角蛋白层进行了重复测量，以确定内包皮和外包皮之间是否存在差异。成人包皮是从伊利诺伊州芝加哥因不明医学指征接受选择性男性包皮环切术的同意捐献者那里收集的。使用抗聚丝蛋白荧光抗体对样本进行处理、切片和角蛋白染色。用 H&E 染色的载玻片用作对照，与之前发表的使用该方法的研究进行比较。以标准化方式测量每个样本的角蛋白层。将我们基于荧光的分析与之前发表的免疫组织化学方法进行比较表明，我们的方法对于测量包皮角蛋白厚度非常准确。不同供体内部和之间的男性包皮内部和外部的角蛋白厚度存在显着的异质性。内外包皮角蛋白厚度无显着差异（分别为25.37±12.51和20.54±12.51μm，p=0.451）。我们发现成年男性包皮内部和外部的角化没有差异。角蛋白层本身不太可能解释为什么未受割礼的男性感染艾滋病毒的风险更高。
The theory that a more thinly keratinized inner foreskin leads to increased HIV-1 susceptibility has been based on relatively little published data. We sought to quantify the keratin thicknesses of the inner and outer foreskin to determine the plausibility of this hypothesis. We took repeated measurements of the keratin layer of 16 adult male foreskins to determine if differences existed between the inner and outer foreskin. Adult foreskins were collected from consenting donors undergoing elective male circumcision for unknown medical indications in Chicago, IL. Specimens were processed, sectioned and stained for keratin using anti-filaggrin fluorescent antibodies. Slides stained with H&E were used as controls to compare to previously published studies using this method. Keratin layers were measured in a standardized fashion for each specimen. Comparing our fluorescence-based analysis to previously published immunohistochemical methods revealed that our method was highly accurate for measuring foreskin keratin thickness. There was significant heterogeneity in the keratin thickness of the inner and outer aspects of the male foreskin within and between the different donors. There was no significant difference between the inner and outer foreskin keratin thickness (25.37 ± 12.51 and 20.54 ± 12.51μm respectively, p=0.451). We found no difference between the keratinization of the inner and outer aspects of the adult male foreskin. Keratin layers alone are unlikely to explain why uncircumcised men are at higher risk for HIV infection.