Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells
Resolvin E1 in Follicular Fluid Acts as a Potential Biomarker and Improves Oocyte Developmental Competence by Optimizing Cumulus Cells
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DOI：
10.3389/fendo.2020.00210
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发表时间：
2020-04-16
影响因子：
5.2
通讯作者：
Sun, Xiaoxi
中科院分区：
文献类型：
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作者：
Zhang, Yijing;Zhu, Zhongyi;Sun, Xiaoxi
卵泡液（FF）的代谢谱已被研究，以寻找卵母细胞质量的生物标志物。Resolvin E1（RvE 1）是一种有效的促消退介质，据报道在细胞功能中具有保护作用。本研究旨在检测RvE 1对卵母细胞质量的预测价值，并探讨RvE 1提高卵母细胞能力的细胞学机制。80份FF样本的代谢谱显示，A组（根据Gardner囊胚评分系统，囊胚评分>= B3 BC和B3 CB，N = 36）中的RvE 1水平高于B组（囊胚评分<B3 BC和B3 CB，N = 44，P = 0.0018）。受试者工作特征（ROC）曲线分析显示，FF中RvE 1水平低于8.96 pg/ml（AUC：0.75; 95%CI：0.64-0.86; P = 0.00012）可预测卵母细胞质量差，特异性为97.22%，提示RvE 1可作为排除劣质卵母细胞的潜在生物标志物。此外，RvE 1在FF中的水平显著低于血清（57.49 - 17.62 pg/ml; P= 0.0037），并且在细胞培养过程中在卵丘细胞（CC）的培养液中逐渐积累，这表明RvE 1来自血液渗出物和局部分泌物。体外实验揭示了RvE 1通过降低卵丘细胞凋亡率、提高细胞活力和增殖能力来提高卵母细胞质量的细胞学机制。这是首次探讨RvE 1在生殖中的作用。结论：RvE 1是一种有价值的卵母细胞选择的特异性生物标志物，在提高卵母细胞质量方面发挥着重要作用。
Metabolic profile of follicular fluid (FF) has been investigated to look for biomarkers for oocyte quality. Resolvin E1 (RvE1), a potent pro-resolving mediator, was reported to have protective action in cell function. The study aimed to examine the predictive value of RvE1 for oocyte quality and to explore the cellular mechanism of RvE1 in improving oocyte competence. Metabolic profiles of 80 FF samples showed a higher level of RvE1 in group A (blastocysts scored >= B3BC and B3CB according to Gardner's blastocyst scoring system, N = 36) than that of group B (blastocysts scored < B3BC and B3CB, N = 44, P = 0.0018). The receiver operating characteristic (ROC) curve analysis showed that RvE1 level in FF below 8.96 pg/ml (AUC:0.75; 95%CI: 0.64-0.86; P = 0.00012) could predict poor oocyte quality with specificity of 97.22%, suggesting RvE1 as a potential biomarker to exclude inferior oocytes. Besides, the level of RvE1 was found to be significantly lower in FF than in serum (57.49 to 17.62 pg/ml; P=.0037) and was gradually accumulated in the culture medium of cumulus cells (CCs) during cell culture, which indicated that RvE1 came from both blood exudates and local secretion. The in vitro experiment revealed thecellular mechanism of RvE1 in improvingoocyte qualityby decreasing the cumulus cellapoptotic rate and increasing cell viability and proliferation. It is the first time thatthe role of RvE1 in reproduction is explored. In conclusion, RvE1 is valuable as a potential exclusive biomarker for oocyte selection andplays a role in improving oocyte quality.