X-irradiation of the Contusion Site Improves Locomotor and Histological Outcomes in Spinal Cord-Injured Rats
X-irradiation of the Contusion Site Improves Locomotor and Histological Outcomes in Spinal Cord-Injured Rats
复制标题
DOI：
10.1006/exnr.2001.7803
复制
发表时间：
2001-11
影响因子：
5.3
通讯作者：
R. Zeman;Yong Feng;Hong Peng;P. Visintainer;C. Moorthy;W. Couldwell;J. D. Etlinger
中科院分区：
文献类型：
--
作者：
R. Zeman;Yong Feng;Hong Peng;P. Visintainer;C. Moorthy;W. Couldwell;J. D. Etlinger
我们已经确定了损伤部位的x射线照射是否可以对抗脊髓挫伤后的组织损失并改善运动功能的恢复。用减重装置对T10水平的大鼠造成挫伤损伤。分别于伤后20分钟和1、2、4、7、17天对损伤部位进行局部x射线照射（20 Gy）。然后用21分Basso， Beattie, and Bresnahan （BBB）量表测定运动恢复情况。在随后的6周观察期间，在挫伤后20分钟和1或2天给予x射线照射（最终BBB评分约为7-8），增强了运动功能的恢复。与未照射大鼠相比，损伤后4-17天的x射线照射对最终运动评分没有显著影响（最终BBB评分约为2），这与先前仅在17-18天进行x射线照射对横断损伤有益的研究形成鲜明对比。恢复的程度与挫伤中心脊髓组织的保留程度直接相关。由于挫伤的治疗时间窗比横断损伤的报道更早，结果表明，挫伤迅速启动潜在的辐射敏感过程，这些过程仅在横断损伤后延迟数周后发生。进一步优化x射线治疗可能会导致脊髓挫伤损伤的有效治疗方式。
We have determined whether X-irradiation of the injury site can oppose tissue loss and improve recovery of locomotor function following contusion injury of the spinal cord. Contusion injury was produced in rats at the level of T10 with a weight drop device. Localized X-irradiation (20 Gy) of the injury site was performed at 20 min and 1, 2, 4, 7, and 17 days postinjury. Locomotor recovery was then determined with the 21-point Basso, Beattie, and Bresnahan (BBB) scale. X-irradiation enhanced recovery of locomotor function during a subsequent 6-week observation period when administered 20 min and 1 or 2 days following contusion injury (final BBB score approximately 7-8). X-irradiation at 4-17 days postinjury did not significantly affect final locomotor scores compared with unirradiated rats (final BBB score approximately 2), in marked contrast to previous studies where X-irradiation applied only at 17-18 days benefitted transection injury. The extent of recovery was directly related to measurements of sparing of spinal cord tissue at the contusion center. Because the treatment time window occurred earlier in contusion than reported for transection injury, the results suggest that contusion injury rapidly initiates underlying radiation-sensitive processes that occur only following a delay of several weeks after transection injury. Further optimization of X-ray treatment may lead to a useful therapeutic modality for use in spinal cord contusion injury.