Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray
Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray
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DOI：
10.1007/s00449-008-0292-7
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发表时间：
2009-08-01
影响因子：
3.8
通讯作者：
Shimizu, Hiroshi
中科院分区：
文献类型：
--
作者：
Dinh, Thai Nho;Nagahisa, Keisuke;Shimizu, Hiroshi
在工业生产过程中，酵母细胞会受到乙醇胁迫，从而影响细胞的生长和生产能力。因此，研究高浓度乙醇下酵母细胞的胞内状态是重要的。在这项研究中，使用DNA微阵列分析，我们进行了全面的表达谱的两个菌株的酿酒酵母，即，在乙醇胁迫条件下表现出活跃生长的乙醇适应菌株及其亲株作为对照。通过比较这两株菌在乙醇胁迫条件下的表达谱，发现乙醇适应菌株中核糖体蛋白相关基因表达量显著上调。此外，线粒体中ATP合成相关基因被认为对乙醇胁迫下的生长很重要。我们希望这些结果能为酵母菌的乙醇耐受性提供更好的理解。
In industrial process, yeast cells are exposed to ethanol stress that affects the cell growth and the productivity. Thus, investigating the intracellular state of yeast cells under high ethanol concentration is important. In this study, using DNA microarray analysis, we performed comprehensive expression profiling of two strains of Saccharomyces cerevisiae, i.e., the ethanol-adapted strain that shows active growth under the ethanol stress condition and its parental strain used as the control. By comparing the expression profiles of these two strains under the ethanol stress condition, we found that the genes related to ribosomal proteins were highly up-regulated in the ethanol-adapted strain. Further, genes related to ATP synthesis in mitochondria were suggested to be important for growth under ethanol stress. We expect that the results will provide a better understanding of ethanol tolerance of yeast.