Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
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DOI：
10.1007/978-1-60761-232-2_11
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Hirohashi, Setsuo
中科院分区：
文献类型：
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作者：
Kondo, Tadashi;Hirohashi, Setsuo
双向差异凝胶电泳（2D-DIGE）是双向聚丙烯酰胺凝胶电泳（2D-PAGE）的高级变体;蛋白质样品用不同的荧光染料标记，混合并通过2D-PAGE分离。2D-DIGE解决了2D-PAGE的主要固有缺点，在生物标志物研究中显示出巨大的实用性。生物标志物的开发需要定量、可重复、高灵敏度和高通量的实验平台，而2D-DIGE符合这些标准。在这里，我们展示了2D-DIGE的优势，并讨论了2D-DIGE提供进一步，更全面的蛋白质组研究的可能性。
Two-dimensional difference gel electrophoresis (2D-DIGE) is an advanced variation of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE); protein samples are labeled with different fluorescent dyes, mixed and separated by 2D-PAGE. 2D-DIGE solves major inherent drawbacks of 2D-PAGE, demonstrating great utility in biomarker studies. Biomarker development requires quantitative, reproducible, highly sensitive and high-throughput experimental platforms, and 2D-DIGE meets these criteria. Here we demonstrate the advantages of 2D-DIGE and discuss the possibilities 2D-DIGE offers for further, more comprehensive proteome studies.