Expression and localisation of dynamin and syntaxin during neural development and neuromuscular synapse formation
Expression and localisation of dynamin and syntaxin during neural development and neuromuscular synapse formation
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神经发育和神经肌肉突触形成过程中动力和突触蛋白的表达和定位
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
W. Phillips
中科院分区：
文献类型：
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作者：
P. G. Noakes;D. Chin;S. S. Kim;S. Liang;W. Phillips
在小鼠胚胎运动神经元发育和神经肌肉突触发生期间检查了动力蛋白和突触蛋白的表达和亚细胞定位。动力蛋白和突触蛋白都可以在胚胎第 10 天（E10；轴突生长前 2 天）和所有随后检查的年龄的脊髓中通过免疫印迹检测到。逆转录和聚合酶链反应 (RT-PCR) 鉴定出早在 E10 时期脊髓中动力蛋白 I 的所有三种羧基末端剪接形式均处于低水平。在脊髓神经元成熟期间，从 E10 到出生后第一天 (P0)，相对于动力 II mRNA，动力 I 的短羧基末端剪接形式 (动力 I*b) 上调，动力 III 也是如此。在突触形成开始后 12 小时内以及整个后续发育过程中，突触融合蛋白免疫染色与神经肌肉突触处的突触小泡蛋白 SV2 共定位。相比之下，动力对于活动依赖性突触小泡回收以及持续的神经传递非常重要，但直到突触形成几天后才能在大多数新形成的突触中检测到。因此，突触处动力的延迟出现预示着神经肌肉接头处强突触传递的新生儿发育。 J.Comp。内罗尔。 410:531–540，1999。© 1999 Wiley-Liss, Inc.
The expression and subcellular localisation of dynamin and syntaxin were examined during the periods of motor neuron development and neuromuscular synaptogenesis in the mouse embryo. Both dynamin and syntaxin could be detected by immunoblotting in the spinal cord at embryonic day 10 (E10; 2 days before axon outgrowth) and at all subsequent ages examined. Reverse transcription and polymerase chain reaction (RT‐PCR) identified low levels of all three carboxy‐terminal splicing forms of dynamin I in spinal cord from as early as E10. During the period of maturation of spinal neurons, from E10 to the first postnatal day (P0), the short carboxy‐terminal splicing form of dynamin I (dynamin I*b) was up‐regulated, as was dynamin III, relative to dynamin II mRNA. Syntaxin immunostaining became colocalized with the synaptic vesicle protein, SV2, at neuromuscular synapses within 12 hours of the commencement of synapse formation and throughout subsequent development. In contrast, dynamin, which is important for activity‐dependent synaptic vesicle recycling and, thus, sustained neurotransmission, could not be detected at most newly formed synapses until several days after synapse formation. The delayed appearance of dynamin at the synapse, thus, heralds the neonatal development of robust synaptic transmission at the neuromuscular junction. J. Comp. Neurol. 410:531–540, 1999. © 1999 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.94.16.8569
发表时间：
1997-08-05
影响因子：
11.1
作者：
Ringstad, N;Nemoto, Y;DeCamilli, P
通讯作者：
DeCamilli, P