Simultaneous determination of plasma indole and skatole in pregnant women with hepatitis B virus infection by high performance liquid chromatography
Simultaneous determination of plasma indole and skatole in pregnant women with hepatitis B virus infection by high performance liquid chromatography
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DOI：
10.3724/sp.j.1123.2017.03025
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发表时间：
2017-07-01
影响因子：
0.7
通讯作者：
Xu Biao
中科院分区：
文献类型：
--
作者：
Deng Linlin;Zhen Qianna;Xu Biao
建立了同时测定血浆中吲哚和粪臭素的高效液相色谱-荧光检测法。血浆样品经液体预处理。液相萃取在Shim-Pack VP-ODS柱（150 mm × 4. 6毫米，4. 6 μ m），使用15 mmol/L磷酸二氢钠溶液和甲醇（40：60，v/v）的等度混合物。荧光激发和发射波长分别为274 nm和340 nm。线性范围为2. 22-88. 89 μ g/L吲哚和1. 11-44.粪臭素为44 μ g/L。检出限为0。11 μ g/L和0. 06 μ g/L。回收率在95。5%-112。3%，相对标准偏差小于6。百分之八该方法已成功地应用于健康孕妇（n = 46）和孕妇与B病毒（HBV）感染（n = 29）的血浆分析。结果表明，plas. ma吲哚和粪臭素水平在两组之间有显著差异。在HBV感染的孕妇中，吲哚类化合物的浓度与转氨酶水平呈正相关。
A high performance liquid chromatographic (HPLC) method with fluorescence detection (FD) was established to simultaneously determine plasma indole and skatole. Plasma samples were pretreated by liquid. liquid extraction. Chromatographic separation was accomplished on a Shim-Pack VP-ODS column (150 mmx4. 6 mm, 4. 6 mu m) using an isocratic mixture of 15 mmol/L sodium dihydrogen phosphate solution and methanol (40:60, v/v). The fluorescence excitation and emission wavelengths were 274 nm and 340 nm, respectively. The linear ranges were 2. 22-88. 89 mu g/L for indole and 1. 11-44. 44 mu g/L for skatole. The detection limits were 0. 11 mu g/L and 0. 06 mu g/L for indole and skatole, respectively. The recoveries were in the range of 95. 5% -112. 3% with the relative standard deviations less than 6. 8%. The method was successfully applied to the analysis of plasma from healthy pregnant women (n = 46) and pregnant women with hepatitis B virus (HBV) infection (n = 29). The results showed that plas. ma indole and skatole levels were significantly different between two groups. In pregnant women with HBV infection, the concentrations of indolic compounds were positively associated with transaminase levels.