Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain
Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain
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DOI：
10.1111/j.1460-9568.2007.05883.x
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发表时间：
2007-11-01
影响因子：
3.4
通讯作者：
Aloe, Luigi
中科院分区：
文献类型：
--
作者：
Di Fausto, Veronica;Fiore, Marco;Aloe, Luigi
我们最近发现，结膜应用神经生长因子（NGF）在大鼠可以达到视网膜，视神经和中枢神经系统。在本研究中，我们调查是否神经生长因子应用作为洗眼液可以促进化学损伤的基底前脑胆碱能神经元的恢复。对先前用鹅膏蕈氨酸静脉注射以损害胆碱能途径的成年雄性小鼠的眼睛给予NGF。在被动回避测试中测试小鼠，并且在施用NGF 2周后杀死小鼠，并将脑用于结构、生物化学和分子分析。结果表明，在眼表面应用NGF保护胆碱乙酰转移酶水平。这些发现加强了这样一种假设，即在眼睛上应用NGF可以代表一种替代的递送途径，以促进变性过程中脑细胞的恢复，包括参与学习和记忆活动的神经元。
We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.