Cell Surface Vimentin Is an Attachment Receptor for Enterovirus 71
Cell Surface Vimentin Is an Attachment Receptor for Enterovirus 71
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细胞表面波形蛋白是肠道病毒 71 的附着受体
DOI：
10.1128/jvi.03826-13
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发表时间：
2014-05-01
影响因子：
5.4
通讯作者：
Tien, Po
中科院分区：
文献类型：
--
作者：
Du, Ning;Cong, Haolong;Tien, Po
摘要肠道病毒71型(EV71)是一种高度传染性的病原体，可引起婴幼儿严重的中枢神经系统疾病。在这里，我们报道了EV71VP1蛋白可以与宿主细胞表面表达的波形蛋白中间丝结合。可溶性波形蛋白或波形蛋白抗体可抑制EV71与宿主细胞的结合。随着细胞表面Vimentin表达的减少，EV71与细胞的结合力显著降低。进一步的证据表明，Vimentin的N端负责EV71与Vimentin之间的相互作用。这些结果表明，宿主细胞表面的波形蛋白可能是介导EV71最初结合并随后增加其感染力的附着部位。重要性这项研究就波形蛋白细丝在EV71感染中的作用提供了重要的发现，并提供了信息，不仅提高了我们对EV71致病机理的理解，还为我们提供了潜在的治疗EV71感染引起的疾病的新策略。
ABSTRACT Enterovirus 71 (EV71) is a highly transmissible pathogenic agent that causes severe central nervous system diseases in infected infants and young children. Here, we reported that EV71 VP1 protein could bind to vimentin intermediate filaments expressed on the host cell surface. Soluble vimentin or an antibody against vimentin could inhibit the binding of EV71 to host cells. Accompanied with the reduction of vimentin expression on the cell surface, the binding of EV71 to cells was remarkably decreased. Further evidence showed that the N terminus of vimentin is responsible for the interaction between EV71 and vimentin. These results indicated that vimentin on the host cell surface may serve as an attachment site that mediated the initial binding and subsequently increased the infectivity of EV71. IMPORTANCE This study delivers important findings on the roles of vimentin filaments in relation to EV71 infection and provides information that not only improves our understanding of EV71 pathogenesis but also presents us with potentially new strategies for the treatment of diseases caused by EV71 infections.