Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.
Processing of unstable bacteriophage T4 gene 32 mRNAs into a stable species requires Escherichia coli ribonuclease E.
复制标题
将不稳定的噬菌体 T4 基因 32 mRNA 加工成稳定的物种需要大肠杆菌核糖核酸酶 E。
作者：
E. A. Mudd;P. Prentki;D. Belin;H. Krisch
来自噬菌体T4的基因32被转录为前体转录物，其被加工成稳定的产物。在大肠杆菌的RNase III或P缺陷菌株中观察到基因32 mRNA的这种加工。然而，在感染RNase E缺陷型菌株后，加工的转录物的量显著减少，而前体转录物的水平仍然很高。因此，RNase E似乎在基因32 mRNA的加工中具有重要作用。我们已经通过引物延伸定位了加工转录物的确切5′端。切割发生在茎环结构附近的一个位点，该位点与其他已知的RNase E切割位点有一定的相似性。的上游和下游序列的差分稳定性，并对基因表达的处理的影响，进行了讨论。
Gene 32 from bacteriophage T4 is transcribed as precursor transcripts which are processed to a stable product. This processing of the gene 32 mRNA was observed in RNase III or P‐deficient strains of Escherichia coli. However, after infection of an RNase E‐deficient strain, the amount of processed transcript was significantly reduced while the levels of the precursor transcripts remained high. RNase E therefore appears to have an essential role in the processing of the gene 32 mRNA. We have mapped the exact 5′ end of the processed transcript by primer extension. The cleavage occurs near a stem‐loop structure at a site which shows some similarity to other known RNase E cleavage sites. The effects of the processing on the differential stability of the upstream and downstream sequences, and on gene expression, are discussed.