Meta-analysis Reveals Potential Influence of Oxidative Stress on the Airway Microbiomes of Cystic Fibrosis Patients
Meta-analysis Reveals Potential Influence of Oxidative Stress on the Airway Microbiomes of Cystic Fibrosis Patients
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DOI：
10.1016/j.gpb.2018.03.009
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发表时间：
2019-12
期刊：
影响因子：
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通讯作者：
Xing Shi;Zhancheng Gao;Qiang Lin;Liping Zhao;Q. Ma;Yu Kang;Jun Yu
中科院分区：
文献类型：
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作者：
Xing Shi;Zhancheng Gao;Qiang Lin;Liping Zhao;Q. Ma;Yu Kang;Jun Yu
囊性纤维化（CF）患者的致死性慢性气道感染易由特定的CF嗜性病原体或CF微生物组的定殖引起，但患者中微生物组定居的关键过程和原因尚未完全了解，特别是其从正常到患病状态的生存和代谢动力学。在这里，我们报告我们的荟萃分析结果CF气道微生物基于代谢网络重建基因组信息在物种水平。CF患者的微生物组似乎比对照组参与更多的氧化还原相关活动，并且通过构建抗氧化应激（抗OS）基因的大型数据集，我们对CF微生物组中每种细菌物种的抗OS能力的定量评估证实了属内抗OS应答的强烈保守性，并且还显示CF病原体具有显著更高的抗-OS应答。OS的能力远高于呼吸道细菌和其他典型的呼吸道病原体。此外，相关种属的抗OS能力与其对CF患者气道的相对适合度相关，而对对照气道的相对适合度相关。此外，CF患者的呼吸道微生物组的总抗OS能力总体上高于对照组，其随着疾病进展而增加，特别是在急性加重和抗生素治疗后。根据这些结果，我们提出CF患者气道中OS的增加可能在重塑气道微生物组以使其更耐药状态中起重要作用，该状态有利于CF病原体的感染前定殖以获得更高的抗OS能力。
The lethal chronic airway infection of thecystic fibrosis(CF) patients is predisposed by colonization of specific CF-philic pathogens or the CF microbiomes, but key processes and reasons of the microbiome settlement in the patients are yet to be fully understood, especially their survival and metabolic dynamics from normal to diseased status under treatment. Here, we report ourmeta-analysisresults on CFairway microbiomesbased on metabolic networks reconstructed from genome information at species level. The microbiomes of CF patients appear to engage much more redox-related activities than those of controls, and by constructing a large dataset of anti-oxidative stress (anti-OS) genes, our quantitative evaluation of the anti-OS capacity of each bacterial species in the CF microbiomes confirms strong conservation of the anti-OS responses within genera and also shows that the CF pathogens have significantly higher anti-OS capacity than commensals and other typicalrespiratory pathogens. In addition, the anti-OS capacity of a relevant species correlates with its relative fitness for the airways of CF patients over that for the airways of controls. Moreover, the total anti-OS capacity of the respiratory microbiome of CF patients is collectively higher than that of controls, which increases with disease progression, especially after episodes of acute exacerbation and antibiotic treatment. According to these results, we propose that the increased OS in the airways of CF patients may play an important role in reshaping airway microbiomes to a more resistant status that favors the pre-infection colonization of the CF pathogens for a higher anti-OS capacity.