Cloning and characterization of DNA complementary to the canine distemper virus mRNA encoding matrix, phosphoprotein, and nucleocapsid protein
Cloning and characterization of DNA complementary to the canine distemper virus mRNA encoding matrix, phosphoprotein, and nucleocapsid protein
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与犬瘟热病毒 mRNA 编码基质、磷蛋白和核衣壳蛋白互补的 DNA 的克隆和表征
DOI：
10.1128/jvi.53.2.691-694.1985
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发表时间：
1985
影响因子：
5.4
通讯作者：
W. Bellini
中科院分区：
文献类型：
--
作者：
S. Rozenblatt;O. Eizenberg;G. Englund;W. Bellini
从感染犬瘟热病毒（CDV）的猴肾细胞中提取含总聚腺苷酸的mRNA合成双链cDNA，并将其克隆到大肠杆菌质粒pBR322的PstI位点。将含有犬瘟热病毒DNA的克隆与犬瘟热病毒特异性的32p标记cDNA杂交鉴定。已经鉴定出包含不同种类序列的四个特定克隆。克隆的质粒包含800（克隆44-80）、960（克隆74-16）、1700（克隆364）和950（克隆40-9）碱基对的插入。根据Northern技术测定，与这些插入物互补的mRNA物种的大小分别为1,500,1,850,1,850和2,500个核苷酸。其中3个克隆的DNA片段被进一步鉴定为CDV基质、磷酸化蛋白和核衣壳蛋白mRNA编码的逆转录本。
Double-stranded cDNA synthesized from total polyadenylate-containing mRNA, extracted from monkey kidney cells infected with canine distemper virus (CDV), has been cloned into the PstI site of Escherichia coli plasmid pBR322. Clones containing canine distemper virus DNA were identified by hybridization to a canine distemper virus-specific, 32P-labeled cDNA. Four specific clones containing different classes of sequences have been identified. The cloned plasmids contain inserts of 800 (clone 44-80), 960 (clone 74-16), 1,700 (clone 364), and 950 (clone 40-9) base pairs. The sizes of the mRNA species complementary to these inserts are 1,500, 1,850, 1,850 and 2,500 nucleotides, respectively, as determined by the Northern technique. Three of the cloned DNA fragments were further identified as the reverse transcripts of the mRNA coding for the matrix, phosphoprotein, and nucleocapsid protein of CDV.